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验证数据展示

  • WB analysis using 84296-4-RR (same clone as 84296-4-PBS)

    WB analysis using 84296-4-RR (same clone as 84296-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84296-4-RR (ARFGEF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84296-4-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 84296-4-RR (same clone as 84296-4-PBS)

    IHC staining of human stomach cancer using 84296-4-RR (same clone as 84296-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84296-4-RR (ARFGEF2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84296-4-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 84296-4-RR (same clone as 84296-4-PBS)

    IHC staining of human stomach cancer using 84296-4-RR (same clone as 84296-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84296-4-RR (ARFGEF2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84296-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01199-3

    Cytometric bead array standard curve of MP01199-3

    Cytometric bead array standard curve of MP01199-3, ARFGEF2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84296-5-PBS. Detection antibody: 84296-4-PBS. Standard: Ag35396. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 84296-4-RR

    Affinity and Kinetic Characterization of 84296-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84296-4-RR against Human ARFGEF2 were performed. The affinity constant is 0.788 nM.

ARFGEF2 Recombinant antibody, PBS Only (Detector)

ARFGEF2 Uni-rAbTM Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 84296-4-PBS

产品说明书

CloneNo. 241538H5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Cytometric bead array, Indirect ELISA

别名

BIG2, Brefeldin A inhibited GEP 2, ARFGEP2, 241538H5

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84296-4-PBS targets ARFGEF2 as part of a matched antibody pair:

MP01199-3: 84296-5-PBS capture and 84296-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 ARFGEF2 fusion protein Ag35396 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited)
别名 BIG2, Brefeldin A inhibited GEP 2, ARFGEP2, 241538H5
观测分子量202 kDa
GenBank蛋白编号BC050449
基因名称 ARFGEF2
Gene ID (NCBI) 10564
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y6D5
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. ARFGEF2 is involved in the activation of ARFs by accelerating replacement of bound GDP with GTP and is involved in Golgi transport. ARFGEF2 contains a Sec7 domain, which may be responsible for its guanine-nucleotide exchange activity and also brefeldin A inhibition.