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POTEA-Specific Recombinant antibody

POTEA-Specific Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241187F7

Cat no : 84298-1-RR

Print datasheet

Synonyms

POTEA, POTE8, POTE 8, ANKRD26-like family A member 1, ANKRD26 like family A member 1

验证数据展示

  • IHC staining of mouse ovary using 84298-1-RR

    IHC staining of mouse ovary using 84298-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 84298-1-RR (POTEA-Specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 84298-1-RR

    IF Staining of HepG2 using 84298-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using POTEA-Specific antibody (84298-1-RR, Clone: 241187F7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of HeLa using 84298-1-RR

    FC experiment of HeLa using 84298-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug POTEA-Specific Recombinant antibody (84298-1-RR, Clone:241187F7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HeLa using 84298-1-RR

    IF Staining of HeLa using 84298-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using POTEA-Specific antibody (84298-1-RR, Clone: 241187F7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive IHC detected inmouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84298-1-RR targets POTEA-Specific in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name POTE ankyrin domain family, member A
Synonyms POTEA, POTE8, POTE 8, ANKRD26-like family A member 1, ANKRD26 like family A member 1
Calculated Molecular Weight 56 kDa
GenBank Accession NumberNM_001002920
Gene Symbol POTEA
Gene ID (NCBI) 340441
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ6S8J7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

POTEA, also named A26A1 and POTE8, belongs to the POTE family. POTE has an important signaling function in the reproductive system.

实验方案

Product Specific Protocols
IHC protocol for POTEA-Specific antibody 84298-1-RRDownload protocol
IF protocol for POTEA-Specific antibody 84298-1-RRDownload protocol
FC protocol for POTEA-Specific antibody 84298-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols