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验证数据展示

  • FC experiment of HeLa using CL488-84298

    FC experiment of HeLa using CL488-84298

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Coralite® Plus 488 POTEA-Specific Recombinant Antibody (CL488-84298, Clone:241187F7)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer.

CoraLite® Plus 488-conjugated POTEA-Specific Recombinant antibody

POTEA-Specific Recombinant Antibody for FC (Intra)
Cat No. CL488-84298
CloneNo. 241187F7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

POTEA, POTE8, POTE 8, ANKRD26-like family A member 1, ANKRD26 like family A member 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 84298-1-PBS

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-84298 targets POTEA-Specific in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name POTE ankyrin domain family, member A
Synonyms POTEA, POTE8, POTE 8, ANKRD26-like family A member 1, ANKRD26 like family A member 1
Calculated Molecular Weight 56 kDa
GenBank Accession NumberNM_001002920
Gene Symbol POTEA
Gene ID (NCBI) 340441
RRIDAB_3673352
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6S8J7
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

POTEA, also named A26A1 and POTE8, belongs to the POTE family. POTE has an important signaling function in the reproductive system.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 POTEA-Specific antibody CL488-84298Download protocol
Standard Protocols
Click here to view our Standard Protocols