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验证数据展示

  • IHC staining of mouse ovary using 84298-1-RR

    IHC staining of mouse ovary using 84298-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 84298-1-RR (POTEA-Specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 84298-1-RR

    IF Staining of HepG2 using 84298-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using POTEA-Specific antibody (84298-1-RR, Clone: 241187F7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of HeLa using 84298-1-RR

    FC experiment of HeLa using 84298-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug POTEA-Specific Recombinant antibody (84298-1-RR, Clone:241187F7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HeLa using 84298-1-RR

    IF Staining of HeLa using 84298-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using POTEA-Specific antibody (84298-1-RR, Clone: 241187F7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

POTEA-Specific Recombinant antibody

POTEA-Specific Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 84298-1-RR

产品说明书

CloneNo. 241187F7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, FC (Intra), ELISA

别名

POTEA, POTE8, POTE 8, ANKRD26-like family A member 1, ANKRD26 like family A member 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84298-1-RR targets POTEA-Specific in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 POTE ankyrin domain family, member A
别名 POTEA, POTE8, POTE 8, ANKRD26-like family A member 1, ANKRD26 like family A member 1
计算分子量 56 kDa
GenBank蛋白编号NM_001002920
基因名称 POTEA
Gene ID (NCBI) 340441
RRIDAB_3671839
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ6S8J7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

POTEA, also named A26A1 and POTE8, belongs to the POTE family. POTE has an important signaling function in the reproductive system.

实验方案

Product Specific Protocols
IHC protocol for POTEA-Specific antibody 84298-1-RRDownload protocol
IF protocol for POTEA-Specific antibody 84298-1-RRDownload protocol
FC protocol for POTEA-Specific antibody 84298-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols