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验证数据展示

  • WB analysis using 84378-5-RR (same clone as 84378-5-PBS)

    WB analysis using 84378-5-RR (same clone as 84378-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84378-5-RR (CYP2R1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84378-5-PBS in a different storage buffer formulation.

  • FC experiment of Hela using 84378-5-RR (same clone as 84378-5-PBS)

    FC experiment of Hela using 84378-5-RR (same clone as 84378-5-PBS)

    1x10^6 Hela cells were intracellularly stained with 0.25 ug CYP2R1 Recombinant antibody (84378-5-RR, Clone:241072F11) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84378-5-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01251-2

    Cytometric bead array standard curve of MP01251-2

    Cytometric bead array standard curve of MP01251-2, CYP2R1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84378-3-PBS. Detection antibody: 84378-5-PBS. Standard: Ag12873. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 84378-5-RR

    Affinity and Kinetic Characterization of 84378-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84378-5-RR against Human CYP2R1 were performed. The affinity constant is 0.243 nM.

CYP2R1 Recombinant antibody, PBS Only (Detector)

CYP2R1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 84378-5-PBS

产品说明书

CloneNo. 241072F11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), Cytometric bead array, Indirect ELISA

别名

Vitamin D 25-hydroxylase, Vitamin D 25 hydroxylase, EC:1.14.14.24, 241072F11

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84378-5-PBS targets CYP2R1 as part of a matched antibody pair:

MP01251-2: 84378-3-PBS capture and 84378-5-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 CYP2R1 fusion protein Ag12873 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cytochrome P450, family 2, subfamily R, polypeptide 1
别名 Vitamin D 25-hydroxylase, Vitamin D 25 hydroxylase, EC:1.14.14.24, 241072F11
计算分子量 501 aa, 57 kDa
观测分子量 57 kDa
GenBank蛋白编号BC104909
基因名称 CYP2R1
Gene ID (NCBI) 120227
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ6VVX0
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CYP2R1 is a member of the cytochrome P450 superfamily of enzymes which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids, and other lipids. CYP2R1 primarily functions as a 25-hydroxylase of vitamin D, responsible for converting vitamin D to 25-hydroxyvitamin D (25-OH-D), affecting the production of the active hormone 1,25(OH)2D (PMID: 12867411). CYP2R1 enzyme is a key vitamin D 25-hydroxylase, mutations in CYP2R1 are associated with Rickets vitamin D-dependent 1B(PMID: 25942481, 15128933).