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验证数据展示

  • FC experiment of Hela using 84378-5-RR

    FC experiment of Hela using 84378-5-RR

    1x10^6 Hela cells were intracellularly stained with 0.25 ug CYP2R1 Recombinant antibody (84378-5-RR, Clone:241072F11) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis using 84378-5-RR

    WB analysis using 84378-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84378-5-RR (CYP2R1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84378-5-RR

    Affinity and Kinetic Characterization of 84378-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84378-5-RR against Human CYP2R1 were performed. The affinity constant is 0.243 nM.

CYP2R1 Recombinant antibody

CYP2R1 Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84378-5-RR

Print datasheet

CloneNo. 241072F11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

Vitamin D 25-hydroxylase, Vitamin D 25 hydroxylase, EC:1.14.14.24, 241072F11

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, HuH-7 cells, human placenta tissue
Positive FC (Intra) detected inHela cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84378-5-RR targets CYP2R1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen CYP2R1 fusion protein Ag12873 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cytochrome P450, family 2, subfamily R, polypeptide 1
Synonyms Vitamin D 25-hydroxylase, Vitamin D 25 hydroxylase, EC:1.14.14.24, 241072F11
Calculated Molecular Weight 501 aa, 57 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC104909
Gene Symbol CYP2R1
Gene ID (NCBI) 120227
RRIDAB_3671915
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6VVX0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CYP2R1 is a member of the cytochrome P450 superfamily of enzymes which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids, and other lipids. CYP2R1 primarily functions as a 25-hydroxylase of vitamin D, responsible for converting vitamin D to 25-hydroxyvitamin D (25-OH-D), affecting the production of the active hormone 1,25(OH)2D (PMID: 12867411). CYP2R1 enzyme is a key vitamin D 25-hydroxylase, mutations in CYP2R1 are associated with Rickets vitamin D-dependent 1B(PMID: 25942481, 15128933).

实验方案

Product Specific Protocols
WB protocol for CYP2R1 antibody 84378-5-RRDownload protocol
FC protocol for CYP2R1 antibody 84378-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols