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验证数据展示

  • FC experiment of Hela using 84378-5-RR

    FC experiment of Hela using 84378-5-RR

    1x10^6 Hela cells were intracellularly stained with 0.25 ug CYP2R1 Recombinant antibody (84378-5-RR, Clone:241072F11) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis using 84378-5-RR

    WB analysis using 84378-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84378-5-RR (CYP2R1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84378-5-RR

    Affinity and Kinetic Characterization of 84378-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84378-5-RR against Human CYP2R1 were performed. The affinity constant is 0.243 nM.

CYP2R1 Recombinant antibody

CYP2R1 Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84378-5-RR

产品说明书

CloneNo. 241072F11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

Vitamin D 25-hydroxylase, Vitamin D 25 hydroxylase, EC:1.14.14.24, 241072F11

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, HuH-7 cells, human placenta tissue
Positive FC (Intra) detected inHela cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84378-5-RR targets CYP2R1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 CYP2R1 fusion protein Ag12873 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cytochrome P450, family 2, subfamily R, polypeptide 1
别名 Vitamin D 25-hydroxylase, Vitamin D 25 hydroxylase, EC:1.14.14.24, 241072F11
计算分子量 501 aa, 57 kDa
观测分子量 57 kDa
GenBank蛋白编号BC104909
基因名称 CYP2R1
Gene ID (NCBI) 120227
RRIDAB_3671915
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ6VVX0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CYP2R1 is a member of the cytochrome P450 superfamily of enzymes which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids, and other lipids. CYP2R1 primarily functions as a 25-hydroxylase of vitamin D, responsible for converting vitamin D to 25-hydroxyvitamin D (25-OH-D), affecting the production of the active hormone 1,25(OH)2D (PMID: 12867411). CYP2R1 enzyme is a key vitamin D 25-hydroxylase, mutations in CYP2R1 are associated with Rickets vitamin D-dependent 1B(PMID: 25942481, 15128933).

实验方案

Product Specific Protocols
WB protocol for CYP2R1 antibody 84378-5-RRDownload protocol
FC protocol for CYP2R1 antibody 84378-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols