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CYP2R1 Recombinant antibody, PBS Only (Detector)

CYP2R1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241072F11

Cat no : 84378-5-PBS

Print datasheet

Synonyms

Vitamin D 25-hydroxylase, Vitamin D 25 hydroxylase, EC:1.14.14.24, 241072F11

验证数据展示

  • WB analysis using 84378-5-RR (same clone as 84378-5-PBS)

    WB analysis using 84378-5-RR (same clone as 84378-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84378-5-RR (CYP2R1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84378-5-PBS in a different storage buffer formulation.

  • FC experiment of Hela using 84378-5-RR (same clone as 84378-5-PBS)

    FC experiment of Hela using 84378-5-RR (same clone as 84378-5-PBS)

    1x10^6 Hela cells were intracellularly stained with 0.25 ug CYP2R1 Recombinant antibody (84378-5-RR, Clone:241072F11) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84378-5-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01251-2

    Cytometric bead array standard curve of MP01251-2

    Cytometric bead array standard curve of MP01251-2, CYP2R1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84378-3-PBS. Detection antibody: 84378-5-PBS. Standard: Ag12873. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 84378-5-RR

    Affinity and Kinetic Characterization of 84378-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84378-5-RR against Human CYP2R1 were performed. The affinity constant is 0.243 nM.

产品信息

84378-5-PBS targets CYP2R1 as part of a matched antibody pair:

MP01251-2: 84378-3-PBS capture and 84378-5-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen CYP2R1 fusion protein Ag12873 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cytochrome P450, family 2, subfamily R, polypeptide 1
Synonyms Vitamin D 25-hydroxylase, Vitamin D 25 hydroxylase, EC:1.14.14.24, 241072F11
Calculated Molecular Weight 501 aa, 57 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC104909
Gene Symbol CYP2R1
Gene ID (NCBI) 120227
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6VVX0
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CYP2R1 is a member of the cytochrome P450 superfamily of enzymes which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids, and other lipids. CYP2R1 primarily functions as a 25-hydroxylase of vitamin D, responsible for converting vitamin D to 25-hydroxyvitamin D (25-OH-D), affecting the production of the active hormone 1,25(OH)2D (PMID: 12867411). CYP2R1 enzyme is a key vitamin D 25-hydroxylase, mutations in CYP2R1 are associated with Rickets vitamin D-dependent 1B(PMID: 25942481, 15128933).