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Mouse CEACAM1/CD66a Recombinant antibody, PBS Only (Capture/Detector)

CEACAM1/CD66a Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241856H5

Cat no : 84409-4-PBS

Print datasheet

Synonyms

Biliary glycoprotein D, Biliary glycoprotein 1, BGP-1, Bgp1, Bgp

验证数据展示

  • WB analysis using 84409-4-RR (same clone as 84409-4-PBS)

    WB analysis using 84409-4-RR (same clone as 84409-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84409-4-RR (CEACAM-1/CD66a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84409-4-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01297-2

    Sandwich ELISA standard curve of MP01297-2

    Sandwich ELISA standard curve of MP01297-2, Mouse CEACAM-1/CD66a Recombinant Matched Antibody Pair - PBS only. 84409-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1423. 84409-3-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL

  • Sample test of MP01297-2

    Sample test of MP01297-2

    The mean CEACAM-1/CD66a concentration was determined to be 67.98 ng/mL in mouse liver tissue extract based on a 3.7 mg/mL extract load and 10.51 ng/mL in mouse small intestine tissue extract based on a 1.6 mg/mL extract load.

  • Cytometric bead array standard curve of MP01297-2

    Cytometric bead array standard curve of MP01297-2

    Cytometric bead array standard curve of MP01297-2, MOUSE CEACAM-1/CD66a Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84409-4-PBS. Detection antibody: 84409-3-PBS. Standard: Eg1423. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP01297-3

    Cytometric bead array standard curve of MP01297-3

    Cytometric bead array standard curve of MP01297-3, MOUSE CEACAM1/CD66a Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84409-2-PBS. Detection antibody: 84409-4-PBS. Standard: Eg1423. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 84409-4-RR

    Affinity and Kinetic Characterization of 84409-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84409-4-RR against Mouse CEACAM1/CD66a were performed. The affinity constant is below 1 pM.

产品信息

84409-4-PBS targets CEACAM1/CD66a as part of a matched antibody pair:

MP01297-2: 84409-4-PBS capture and 84409-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP01297-3: 84409-2-PBS capture and 84409-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity mouse
Immunogen FusionProtein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name carcinoembryonic antigen-related cell adhesion molecule 1
Synonyms Biliary glycoprotein D, Biliary glycoprotein 1, BGP-1, Bgp1, Bgp
Calculated Molecular Weight57 kDa
Observed Molecular Weight120 kDa
Gene Symbol Ceacam1
Gene ID (NCBI) 26365
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP31809-1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CEACAM1, also known as CD66a or BGP (biliary glycoprotein), is a member of the cacinoembryonic antigen (CEA) family which belongs to the immunoglobulin superfamily of cell adhesion molecules (PMID: 16919437). CEACAMs play major roles in cell-cell and cell-ECM adhesion and have been implicated in the control of cell proliferation, angiogenesis, and tissue remodeling (PMID: 23021083). CEACAM1 is a transmembrane glycoprotein that is expressed on epithelial, endothelial, and immune cells (PMID: 30314283). CEACAM1 has been demonstrated to play a role in morphogenesis, apoptosis, angiogenesis, cell proliferation, cell motility, fibrosis, and immunity (PMID: 31604530).