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Ki-67 Recombinant antibody

Ki-67 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241768E2

Cat no : 84432-1-RR

Print datasheet

Synonyms

Mki67, Ki67, Ki 67, Proliferation related Ki 67 antigen, 241768E2

验证数据展示

  • WB analysis of NIH/3T3 using 84432-1-RR

    WB analysis of NIH/3T3 using 84432-1-RR

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84432-1-RR (Ki67/MKI67 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of C2C12 using 84432-1-RR

    IF Staining of C2C12 using 84432-1-RR

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Ki-67 antibody (84432-1-RR, Clone: 241768E2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IHC staining of mouse spleen using 84432-1-RR

    IHC staining of mouse spleen using 84432-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84432-1-RR (Ki-67 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 84432-1-RR

    IHC staining of mouse spleen using 84432-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84432-1-RR (Ki-67 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84432-1-RR

    Affinity and Kinetic Characterization of 84432-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84432-1-RR against Mouse Ki-67 were performed. The affinity constant is 3.47 nM.

经过测试的应用

Positive WB detected inNIH/3T3 cells
Positive IHC detected inmouse spleen tissue, rat spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inC2C12 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84432-1-RR targets Ki-67 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity mouse, rat
Immunogen FusionProtein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name antigen identified by monoclonal antibody Ki 67
Synonyms Mki67, Ki67, Ki 67, Proliferation related Ki 67 antigen, 241768E2
Calculated Molecular Weight351kDa
Observed Molecular Weight350 kDa
GenBank Accession NumberNM_001081117.2
Gene Symbol ki67
Gene ID (NCBI) 17345
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDE9PVX6-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry.

实验方案

Product Specific Protocols
WB protocol for Ki-67 antibody 84432-1-RRDownload protocol
IHC protocol for Ki-67 antibody 84432-1-RRDownload protocol
IF protocol for Ki-67 antibody 84432-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols