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验证数据展示

  • IF Staining of C2C12 using 84432-1-RR (same clone as 84432-1-PBS)

    IF Staining of C2C12 using 84432-1-RR (same clone as 84432-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Ki-67 antibody (84432-1-RR, Clone: 241768E2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84432-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 84432-1-RR (same clone as 84432-1-PBS)

    IHC staining of mouse spleen using 84432-1-RR (same clone as 84432-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84432-1-RR (Ki-67 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84432-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 84432-1-RR (same clone as 84432-1-PBS)

    IHC staining of mouse spleen using 84432-1-RR (same clone as 84432-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84432-1-RR (Ki-67 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84432-1-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 84432-1-RR (same clone as 84432-1-PBS)

    WB analysis of NIH/3T3 using 84432-1-RR (same clone as 84432-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84432-1-RR (Ki67/MKI67 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84432-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01277-1

    Cytometric bead array standard curve of MP01277-1

    Cytometric bead array standard curve of MP01277-1, MOUSE Ki-67 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84432-2-PBS. Detection antibody: 84432-1-PBS. Standard: Eg1548. Range: 0.625-40 ng/mL.

Mouse Ki-67 Recombinant antibody, PBS Only (Detector)

Ki-67 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 84432-1-PBS

产品说明书

CloneNo. 241768E2

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

Mki67, Ki67, Ki 67, Proliferation related Ki 67 antigen, 241768E2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84432-1-PBS targets Ki-67 as part of a matched antibody pair:

MP01277-1: 84432-2-PBS capture and 84432-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 mouse, rat
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 antigen identified by monoclonal antibody Ki 67
别名 Mki67, Ki67, Ki 67, Proliferation related Ki 67 antigen, 241768E2
计算分子量351kDa
观测分子量350 kDa
GenBank蛋白编号NM_001081117.2
基因名称 ki67
Gene ID (NCBI) 17345
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDE9PVX6-1
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry.