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验证数据展示

  • WB analysis of NIH/3T3 using 84432-1-RR

    WB analysis of NIH/3T3 using 84432-1-RR

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84432-1-RR (Ki67/MKI67 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of C2C12 using 84432-1-RR

    IF Staining of C2C12 using 84432-1-RR

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Ki-67 antibody (84432-1-RR, Clone: 241768E2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IHC staining of mouse spleen using 84432-1-RR

    IHC staining of mouse spleen using 84432-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84432-1-RR (Ki-67 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 84432-1-RR

    IHC staining of mouse spleen using 84432-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84432-1-RR (Ki-67 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Ki-67 Recombinant antibody

Ki-67 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84432-1-RR

产品说明书

CloneNo. 241768E2

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Mki67, Ki67, Ki 67, Proliferation related Ki 67 antigen, 241768E2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inNIH/3T3 cells
Positive IHC detected inmouse spleen tissue, rat spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inC2C12 cells, NIH/3T3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84432-1-RR targets Ki-67 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 mouse, rat
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 antigen identified by monoclonal antibody Ki 67
别名 Mki67, Ki67, Ki 67, Proliferation related Ki 67 antigen, 241768E2
计算分子量351kDa
观测分子量350 kDa
GenBank蛋白编号NM_001081117.2
基因名称 ki67
Gene ID (NCBI) 17345
RRIDAB_3671959
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDE9PVX6-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry.

实验方案

Product Specific Protocols
WB protocol for Ki-67 antibody 84432-1-RRDownload protocol
IHC protocol for Ki-67 antibody 84432-1-RRDownload protocol
IF protocol for Ki-67 antibody 84432-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols