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GMEB1-Specific Recombinant antibody

GMEB1-Specific Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241447C6

Cat no : 84448-1-RR

Print datasheet

Synonyms

GMEB1, GMEB-1, GMEB 1, Glucocorticoid modulatory element-binding protein 1, DNA-binding protein p96PIF

验证数据展示

  • WB analysis using 84448-1-RR

    WB analysis using 84448-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84448-1-RR (GMEB1-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 84448-1-RR

    FC experiment of HeLa using 84448-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug GMEB1-Specific Recombinant antibody (84448-1-RR, Clone:241447C6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

经过测试的应用

Positive WB detected inHeLa cells, A549 cells, Daudi cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84448-1-RR targets GMEB1-Specific in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name glucocorticoid modulatory element binding protein 1
Synonyms GMEB1, GMEB-1, GMEB 1, Glucocorticoid modulatory element-binding protein 1, DNA-binding protein p96PIF
Calculated Molecular Weight 63 kDa
Observed Molecular Weight88 kDa
GenBank Accession NumberNM_006582
Gene Symbol GMEB1
Gene ID (NCBI) 10691
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9Y692
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GMEB1 is a trans-acting factor that binds to glucocorticoid modulatory elements (GME), acting in estrogen receptor-regulated pathway GREB1 is expressed in proliferating prostate tissue & prostate cancer and is thought to function in hormone-dependent cancer growth. It associates with GMEB2 protein, and the complex is essential for parvovirus DNA replication.

实验方案

Product Specific Protocols
WB protocol for GMEB1-Specific antibody 84448-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols