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ZC3H12A Recombinant antibody

ZC3H12A Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241876H5

Cat no : 84521-5-RR

Print datasheet

Synonyms

MCPIP, MCP-induced protein 1, MCP induced protein 1, Endoribonuclease ZC3H12A, EC:3.1.-.-

验证数据展示

  • WB analysis using 84521-5-RR

    WB analysis using 84521-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84521-5-RR (ZC3H12A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of U-251 using 84521-5-RR

    IF Staining of U-251 using 84521-5-RR

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using ZC3H12A antibody (84521-5-RR, Clone: 241876H5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HeLa using 84521-5-RR

    FC experiment of HeLa using 84521-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug ZC3H12A Recombinant antibody (84521-5-RR, Clone:241876H5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inK-562 cells, Raji cells, HT-1376 cells, MDA-MB-468 cells
Positive IF/ICC detected inU-251 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ZC3H12A Ready-To-Use IHC Kit. ZC3H12A primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84521-5-RR targets ZC3H12A in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen ZC3H12A fusion protein Ag13877 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name zinc finger CCCH-type containing 12A
Synonyms MCPIP, MCP-induced protein 1, MCP induced protein 1, Endoribonuclease ZC3H12A, EC:3.1.-.-
Calculated Molecular Weight 599 aa, 66 kDa
Observed Molecular Weight66 kDa
GenBank Accession NumberBC005001
Gene Symbol ZC3H12A
Gene ID (NCBI) 80149
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ5D1E8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZC3H12A, also named as MCPIP or MCPIP1, is a 599 amino acid protein, which contains one C3H1-type zinc finger and belongs to the ZC3H12 family. ZC3H12A localizes in the cytoplasm and nucleus. Increased expression of ZC3H12A is associated with ischemic heart disease. ZC3H12A has RNase activity and selectively degrades specific target mRNA species and modulates the immune response and inflammation by regulating the decay of specific mRNA molecules.

实验方案

Product Specific Protocols
WB protocol for ZC3H12A antibody 84521-5-RRDownload protocol
IF protocol for ZC3H12A antibody 84521-5-RRDownload protocol
FC protocol for ZC3H12A antibody 84521-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols