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ZC3H12A Recombinant antibody, PBS Only

ZC3H12A Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241876H5

Cat no : 84521-5-PBS

Print datasheet

Synonyms

MCPIP, MCP-induced protein 1, MCP induced protein 1, Endoribonuclease ZC3H12A, EC:3.1.-.-

验证数据展示

  • WB analysis using 84521-5-RR (same clone as 84521-5-PBS)

    WB analysis using 84521-5-RR (same clone as 84521-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84521-5-RR (ZC3H12A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84521-5-PBS in a different storage buffer formulation.

  • IF Staining of U-251 using 84521-5-RR (same clone as 84521-5-PBS)

    IF Staining of U-251 using 84521-5-RR (same clone as 84521-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using ZC3H12A antibody (84521-5-RR, Clone: 241876H5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84521-5-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 84521-5-RR (same clone as 84521-5-PBS)

    FC experiment of HeLa using 84521-5-RR (same clone as 84521-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug ZC3H12A Recombinant antibody (84521-5-RR, Clone:241876H5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84521-5-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy ZC3H12A Ready-To-Use IHC Kit. ZC3H12A primary antibody included.

产品信息

84521-5-PBS targets ZC3H12A in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen ZC3H12A fusion protein Ag13877 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name zinc finger CCCH-type containing 12A
Synonyms MCPIP, MCP-induced protein 1, MCP induced protein 1, Endoribonuclease ZC3H12A, EC:3.1.-.-
Calculated Molecular Weight 599 aa, 66 kDa
Observed Molecular Weight66 kDa
GenBank Accession NumberBC005001
Gene Symbol ZC3H12A
Gene ID (NCBI) 80149
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ5D1E8
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ZC3H12A, also named as MCPIP or MCPIP1, is a 599 amino acid protein, which contains one C3H1-type zinc finger and belongs to the ZC3H12 family. ZC3H12A localizes in the cytoplasm and nucleus. Increased expression of ZC3H12A is associated with ischemic heart disease. ZC3H12A has RNase activity and selectively degrades specific target mRNA species and modulates the immune response and inflammation by regulating the decay of specific mRNA molecules.