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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 84521-5-RR (same clone as 84521-5-PBS)

    WB analysis using 84521-5-RR (same clone as 84521-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84521-5-RR (ZC3H12A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84521-5-PBS in a different storage buffer formulation.

  • IF Staining of U-251 using 84521-5-RR (same clone as 84521-5-PBS)

    IF Staining of U-251 using 84521-5-RR (same clone as 84521-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using ZC3H12A antibody (84521-5-RR, Clone: 241876H5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84521-5-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 84521-5-RR (same clone as 84521-5-PBS)

    FC experiment of HeLa using 84521-5-RR (same clone as 84521-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug ZC3H12A Recombinant antibody (84521-5-RR, Clone:241876H5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84521-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84521-5-RR

    Affinity and Kinetic Characterization of 84521-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84521-5-RR against Human ZC3H12A were performed. The affinity constant is 6.27 nM.

ZC3H12A Recombinant antibody, PBS Only

ZC3H12A Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84521-5-PBS

产品说明书

CloneNo. 241876H5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

MCPIP, MCP-induced protein 1, MCP induced protein 1, Endoribonuclease ZC3H12A, EC:3.1.-.-

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ZC3H12A Ready-To-Use IHC Kit. ZC3H12A primary antibody included.

产品信息

84521-5-PBS targets ZC3H12A in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 ZC3H12A fusion protein Ag13877 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 zinc finger CCCH-type containing 12A
别名 MCPIP, MCP-induced protein 1, MCP induced protein 1, Endoribonuclease ZC3H12A, EC:3.1.-.-
计算分子量 599 aa, 66 kDa
观测分子量66 kDa
GenBank蛋白编号BC005001
基因名称 ZC3H12A
Gene ID (NCBI) 80149
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ5D1E8
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ZC3H12A, also named as MCPIP or MCPIP1, is a 599 amino acid protein, which contains one C3H1-type zinc finger and belongs to the ZC3H12 family. ZC3H12A localizes in the cytoplasm and nucleus. Increased expression of ZC3H12A is associated with ischemic heart disease. ZC3H12A has RNase activity and selectively degrades specific target mRNA species and modulates the immune response and inflammation by regulating the decay of specific mRNA molecules.