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验证数据展示

  • WB analysis using 84581-3-RR

    WB analysis using 84581-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84581-3-RR (TRAF2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 84581-3-RR

    IHC staining of human ovary cancer using 84581-3-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84581-3-RR (TRAF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary cancer using 84581-3-RR

    IHC staining of human ovary cancer using 84581-3-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84581-3-RR (TRAF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 84581-3-RR

    IF Staining of HeLa using 84581-3-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TRAF2 antibody (84581-3-RR, Clone: 241899A2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of HeLa using 84581-3-RR

    FC experiment of HeLa using 84581-3-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug TRAF2 Recombinant antibody (84581-3-RR, Clone:241899A2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84581-3-RR

    Affinity and Kinetic Characterization of 84581-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84581-3-RR against Human TRAF2 were performed. The affinity constant is 0.544 nM.

TRAF2 Recombinant antibody

TRAF2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 84581-3-RR

Print datasheet

CloneNo. 241899A2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

TRAP, TNF receptor-associated factor 2, RING-type E3 ubiquitin transferase TRAF2, EC:2.3.2.27, E3 ubiquitin-protein ligase TRAF2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293T cells, HepG2 cells, MOLT-4 cells. NIH/3T3 cells, C2C12 cells
Positive IHC detected inhuman ovary cancer tissue, human rectal cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84581-3-RR targets TRAF2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse
Immunogen TRAF2 fusion protein Ag25387 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name TNF receptor-associated factor 2
Synonyms TRAP, TNF receptor-associated factor 2, RING-type E3 ubiquitin transferase TRAF2, EC:2.3.2.27, E3 ubiquitin-protein ligase TRAF2
Calculated Molecular Weight 56 kDa
Observed Molecular Weight53 kDa
GenBank Accession NumberBC032410
Gene Symbol TRAF2
Gene ID (NCBI) 7186
RRIDAB_3672080
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ12933
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tumor necrosis factor (TNF) receptor-associated factor-2 (TRAF2) is one of the members of the TRAF superfamily protein, which is an intracellular junction protein with E3 ligase activity. TRAF2 mediates and regulates the activation of nuclear factor kappa B (NF-κB) and microtubule-associated protein kinase (MAPK) signaling pathways by binding to TNFR family proteins. Recent studies have demonstrated that TRAF2 regulates tumor progression by through multiple pathways.

实验方案

Product Specific Protocols
WB protocol for TRAF2 antibody 84581-3-RRDownload protocol
IHC protocol for TRAF2 antibody 84581-3-RRDownload protocol
IF protocol for TRAF2 antibody 84581-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols