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验证数据展示

  • WB analysis using 84588-1-RR

    WB analysis using 84588-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84588-1-RR (MAN1B1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 84588-1-RR

    WB analysis using 84588-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84588-1-RR (MAN1B1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of U2OS using 84588-1-RR

    FC experiment of U2OS using 84588-1-RR

    1x10^6 U2OS cells were intracellularly stained with 0.2 ug MAN1B1 Recombinant antibody (84588-1-RR, Clone:241817E10) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005)(red), or 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

MAN1B1 Recombinant antibody

MAN1B1 Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84588-1-RR

产品说明书

CloneNo. 241817E10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

ER mannosidase 1, ER alpha-1,2-mannosidase, ER alpha 1,2 mannosidase, Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase, EC:3.2.1.113

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, HuH-7 cells, HeLa cells, LNCaP cells, HEK-293 cells, U-87 MG cells
Positive FC (Intra) detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84588-1-RR targets MAN1B1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 MAN1B1 fusion protein Ag28302 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mannosidase, alpha, class 1B, member 1
别名 ER mannosidase 1, ER alpha-1,2-mannosidase, ER alpha 1,2 mannosidase, Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase, EC:3.2.1.113
计算分子量 80 kDa
观测分子量 80 kDa
GenBank蛋白编号BC002953
基因名称 MAN1B1
Gene ID (NCBI) 11253
RRIDAB_3672084
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9UKM7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAN1B1, also known as ERMAN1, MANA-ER, belongs to the glycosyl hydrolase 47 family. MAN1B1 is involved in glycoprotein quality control targeting of misfolded glycoproteins for degradation, specifically converting Man9GlcNAc to Man8GlcNAc isomer B. At high enzyme concentrations, it further trims the carbohydrates to Man5-6GlcNAc2 (PMID: 18003979). Mutations in MAN1B1 cause autosomal-recessive intellectual disability. This gene has been associated with Rafiq syndrome (PMID: 21763484).

实验方案

Product Specific Protocols
WB protocol for MAN1B1 antibody 84588-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols