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MAN1B1 Recombinant antibody, PBS Only

MAN1B1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241817E10

Cat no : 84588-1-PBS

Print datasheet

Synonyms

ER mannosidase 1, ER alpha-1,2-mannosidase, ER alpha 1,2 mannosidase, Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase, EC:3.2.1.113

验证数据展示

  • WB analysis using 84588-1-RR (same clone as 84588-1-PBS)

    WB analysis using 84588-1-RR (same clone as 84588-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84588-1-RR (MAN1B1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84588-1-PBS in a different storage buffer formulation.

  • WB analysis using 84588-1-RR (same clone as 84588-1-PBS)

    WB analysis using 84588-1-RR (same clone as 84588-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84588-1-RR (MAN1B1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84588-1-PBS in a different storage buffer formulation.

  • FC experiment of U2OS using 84588-1-RR (same clone as 84588-1-PBS)

    FC experiment of U2OS using 84588-1-RR (same clone as 84588-1-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.2 ug MAN1B1 Recombinant antibody (84588-1-RR, Clone:241817E10) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005)(red), or 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84588-1-PBS in a different storage buffer formulation.

产品信息

84588-1-PBS targets MAN1B1 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen MAN1B1 fusion protein Ag28302 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name mannosidase, alpha, class 1B, member 1
Synonyms ER mannosidase 1, ER alpha-1,2-mannosidase, ER alpha 1,2 mannosidase, Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase, EC:3.2.1.113
Calculated Molecular Weight 80 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC002953
Gene Symbol MAN1B1
Gene ID (NCBI) 11253
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9UKM7
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MAN1B1, also known as ERMAN1, MANA-ER, belongs to the glycosyl hydrolase 47 family. MAN1B1 is involved in glycoprotein quality control targeting of misfolded glycoproteins for degradation, specifically converting Man9GlcNAc to Man8GlcNAc isomer B. At high enzyme concentrations, it further trims the carbohydrates to Man5-6GlcNAc2 (PMID: 18003979). Mutations in MAN1B1 cause autosomal-recessive intellectual disability. This gene has been associated with Rafiq syndrome (PMID: 21763484).