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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84588-1-RR (same clone as 84588-1-PBS)

    WB analysis using 84588-1-RR (same clone as 84588-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84588-1-RR (MAN1B1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84588-1-PBS in a different storage buffer formulation.

  • WB analysis using 84588-1-RR (same clone as 84588-1-PBS)

    WB analysis using 84588-1-RR (same clone as 84588-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84588-1-RR (MAN1B1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84588-1-PBS in a different storage buffer formulation.

  • FC experiment of U2OS using 84588-1-RR (same clone as 84588-1-PBS)

    FC experiment of U2OS using 84588-1-RR (same clone as 84588-1-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.2 ug MAN1B1 Recombinant antibody (84588-1-RR, Clone:241817E10) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005)(red), or 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84588-1-PBS in a different storage buffer formulation.

MAN1B1 Recombinant antibody, PBS Only

MAN1B1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 84588-1-PBS

产品说明书

CloneNo. 241817E10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), Indirect ELISA

别名

ER mannosidase 1, ER alpha-1,2-mannosidase, ER alpha 1,2 mannosidase, Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase, EC:3.2.1.113

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84588-1-PBS targets MAN1B1 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 MAN1B1 fusion protein Ag28302 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mannosidase, alpha, class 1B, member 1
别名 ER mannosidase 1, ER alpha-1,2-mannosidase, ER alpha 1,2 mannosidase, Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase, EC:3.2.1.113
计算分子量 80 kDa
观测分子量 80 kDa
GenBank蛋白编号BC002953
基因名称 MAN1B1
Gene ID (NCBI) 11253
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9UKM7
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MAN1B1, also known as ERMAN1, MANA-ER, belongs to the glycosyl hydrolase 47 family. MAN1B1 is involved in glycoprotein quality control targeting of misfolded glycoproteins for degradation, specifically converting Man9GlcNAc to Man8GlcNAc isomer B. At high enzyme concentrations, it further trims the carbohydrates to Man5-6GlcNAc2 (PMID: 18003979). Mutations in MAN1B1 cause autosomal-recessive intellectual disability. This gene has been associated with Rafiq syndrome (PMID: 21763484).