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验证数据展示

  • WB analysis using 84602-5-RR

    WB analysis using 84602-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84602-5-RR (CORO1A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of Jurkat using 84602-5-RR

    IF Staining of Jurkat using 84602-5-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CORO1A antibody (84602-5-RR, Clone: 242027H5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of Jurkat using 84602-5-RR

    FC experiment of Jurkat using 84602-5-RR

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug CORO1A Recombinant antibody (84602-5-RR, Clone:242027H5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CORO1A Recombinant antibody

CORO1A Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84602-5-RR

产品说明书

CloneNo. 242027H5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, FC (Intra), ELISA

别名

CORO1, Clipin-A, CLIPINA, Clipin A, CLABP

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, RAW 264.7 cells, mouse brain tissue, rat brain tissue
Positive IF/ICC detected inJurkat cells
Positive FC (Intra) detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy CORO1A Ready-To-Use IHC Kit. CORO1A primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84602-5-RR targets CORO1A in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CORO1A fusion protein Ag12139 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 coronin, actin binding protein, 1A
别名 CORO1, Clipin-A, CLIPINA, Clipin A, CLABP
计算分子量 461 aa, 51 kDa
观测分子量 57 kDa
GenBank蛋白编号BC110374
基因名称 CORO1A
Gene ID (NCBI) 11151
RRIDAB_3672091
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP31146
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CORO1A also known as TACO, belongs to the WD repeat coronin family. CORO1A is recruited to phagosomes and actively retained by surviving mycobacteria and is usually released before phagosome fusion with or maturation into lysosomes. CORO1A is expressed in the brain, thymus, spleen, bone marrow, and lymph nodes. Mutations in CORO1A cause Immunodeficiency 8 with lymphoproliferation (IMD8)(PMID: 10338208).

实验方案

Product Specific Protocols
WB protocol for CORO1A antibody 84602-5-RRDownload protocol
IF protocol for CORO1A antibody 84602-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols