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验证数据展示

  • WB analysis using 84602-5-RR (same clone as 84602-5-PBS)

    WB analysis using 84602-5-RR (same clone as 84602-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84602-5-RR (CORO1A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84602-5-PBS in a different storage buffer formulation.

  • IF Staining of Jurkat using 84602-5-RR (same clone as 84602-5-PBS)

    IF Staining of Jurkat using 84602-5-RR (same clone as 84602-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CORO1A antibody (84602-5-RR, Clone: 242027H5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84602-5-PBS in a different storage buffer formulation.

  • FC experiment of Jurkat using 84602-5-RR (same clone as 84602-5-PBS)

    FC experiment of Jurkat using 84602-5-RR (same clone as 84602-5-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug CORO1A Recombinant antibody (84602-5-RR, Clone:242027H5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84602-5-PBS in a different storage buffer formulation.

CORO1A Recombinant antibody, PBS Only

CORO1A Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84602-5-PBS

产品说明书

CloneNo. 242027H5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

CORO1, Clipin-A, CLIPINA, Clipin A, CLABP

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CORO1A Ready-To-Use IHC Kit. CORO1A primary antibody included.

产品信息

84602-5-PBS targets CORO1A in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CORO1A fusion protein Ag12139 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 coronin, actin binding protein, 1A
别名 CORO1, Clipin-A, CLIPINA, Clipin A, CLABP
计算分子量 461 aa, 51 kDa
观测分子量 57 kDa
GenBank蛋白编号BC110374
基因名称 CORO1A
Gene ID (NCBI) 11151
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP31146
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CORO1A also known as TACO, belongs to the WD repeat coronin family. CORO1A is recruited to phagosomes and actively retained by surviving mycobacteria and is usually released before phagosome fusion with or maturation into lysosomes. CORO1A is expressed in the brain, thymus, spleen, bone marrow, and lymph nodes. Mutations in CORO1A cause Immunodeficiency 8 with lymphoproliferation (IMD8)(PMID: 10338208).