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CORO1A Recombinant antibody

CORO1A Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

242027H5

Cat no : 84602-5-RR

Print datasheet

Synonyms

CORO1, Clipin-A, CLIPINA, Clipin A, CLABP

验证数据展示

  • WB analysis using 84602-5-RR

    WB analysis using 84602-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84602-5-RR (CORO1A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of Jurkat using 84602-5-RR

    IF Staining of Jurkat using 84602-5-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CORO1A antibody (84602-5-RR, Clone: 242027H5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inJurkat cells, RAW 264.7 cells, mouse brain tissue, rat brain tissue
Positive IF/ICC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy CORO1A Ready-To-Use IHC Kit. CORO1A primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84602-5-RR targets CORO1A in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CORO1A fusion protein Ag12139 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name coronin, actin binding protein, 1A
Synonyms CORO1, Clipin-A, CLIPINA, Clipin A, CLABP
Calculated Molecular Weight 461 aa, 51 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC110374
Gene Symbol CORO1A
Gene ID (NCBI) 11151
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP31146
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CORO1A also known as TACO, belongs to the WD repeat coronin family. CORO1A is recruited to phagosomes and actively retained by surviving mycobacteria and is usually released before phagosome fusion with or maturation into lysosomes. CORO1A is expressed in the brain, thymus, spleen, bone marrow, and lymph nodes. Mutations in CORO1A cause Immunodeficiency 8 with lymphoproliferation (IMD8)(PMID: 10338208).

实验方案

Product Specific Protocols
WB protocol for CORO1A antibody 84602-5-RRDownload protocol
IF protocol for CORO1A antibody 84602-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols