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验证数据展示

  • WB analysis using 84656-4-RR

    WB analysis using 84656-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84656-4-RR (RXRB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 84656-4-RR

    IF Staining of MCF-7 using 84656-4-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using RXRB antibody (84656-4-RR, Clone: 242178D4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HeLa using 84656-4-RR

    FC experiment of HeLa using 84656-4-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RXRB Recombinant antibody (84656-4-RR, Clone:242178D4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84656-4-RR

    Affinity and Kinetic Characterization of 84656-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84656-4-RR against Human RXRB were performed. The affinity constant is 1.24 nM.

RXRB Recombinant antibody

RXRB Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84656-4-RR
CloneNo. 242178D4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

Retinoic acid receptor RXR-beta, RCoR 1, NR2B2, H 2RIIBP, DAUDI6

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inSK-BR-3 cells, K-562 cells
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RXRB Ready-To-Use IHC Kit. RXRB primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84656-4-RR targets RXRB in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen RXRB fusion protein Ag0263 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name retinoid X receptor, beta
Synonyms Retinoic acid receptor RXR-beta, RCoR 1, NR2B2, H 2RIIBP, DAUDI6
Calculated Molecular Weight 57 kDa
GenBank Accession NumberBC001167
Gene Symbol RXRB
Gene ID (NCBI) 6257
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP28702
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Retinoid X Receptor beta (RXRB) is a member of the retinoid X receptor (RXR) family of nuclear receptors which are involved in mediating the effects of retinoic acid. RXRB heterodimerizes with other nuclear hormone receptors, such as RAR, the thyroid hormone receptor, and the vitamin D3 receptor, to enhance binding to their cognate response elements. (PMID: 8257090)

实验方案

Product Specific Protocols
WB protocol for RXRB antibody 84656-4-RRDownload protocol
IF protocol for RXRB antibody 84656-4-RRDownload protocol
FC protocol for RXRB antibody 84656-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols