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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84656-4-RR (same clone as 84656-4-PBS)

    WB analysis using 84656-4-RR (same clone as 84656-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84656-4-RR (RXRB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84656-4-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 84656-4-RR (same clone as 84656-4-PBS)

    IF Staining of MCF-7 using 84656-4-RR (same clone as 84656-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using RXRB antibody (84656-4-RR, Clone: 242178D4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84656-4-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 84656-4-RR (same clone as 84656-4-PBS)

    FC experiment of HeLa using 84656-4-RR (same clone as 84656-4-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RXRB Recombinant antibody (84656-4-RR, Clone:242178D4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84656-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84656-4-RR

    Affinity and Kinetic Characterization of 84656-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84656-4-RR against Human RXRB were performed. The affinity constant is 1.24 nM.

RXRB Recombinant antibody, PBS Only

RXRB Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84656-4-PBS
CloneNo. 242178D4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

Retinoic acid receptor RXR-beta, RCoR 1, NR2B2, H 2RIIBP, DAUDI6

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy RXRB Ready-To-Use IHC Kit. RXRB primary antibody included.

产品信息

84656-4-PBS targets RXRB in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen RXRB fusion protein Ag0263 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name retinoid X receptor, beta
Synonyms Retinoic acid receptor RXR-beta, RCoR 1, NR2B2, H 2RIIBP, DAUDI6
Calculated Molecular Weight 57 kDa
GenBank Accession NumberBC001167
Gene Symbol RXRB
Gene ID (NCBI) 6257
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP28702
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Retinoid X Receptor beta (RXRB) is a member of the retinoid X receptor (RXR) family of nuclear receptors which are involved in mediating the effects of retinoic acid. RXRB heterodimerizes with other nuclear hormone receptors, such as RAR, the thyroid hormone receptor, and the vitamin D3 receptor, to enhance binding to their cognate response elements. (PMID: 8257090)