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PSPC1 Recombinant antibody

PSPC1 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

242158G7

Cat no : 84657-4-RR

Print datasheet

Synonyms

PSPC 1, PSP1, 242158G7

验证数据展示

  • WB analysis using 84657-4-RR

    WB analysis using 84657-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84657-4-RR (PSPC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84657-4-RR

    Affinity and Kinetic Characterization of 84657-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84657-4-RR against Human PSPC1 were performed. The affinity constant is 0.68 nM.

经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, HeLa cells, PC-3 cells, MDA-MB-453 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84657-4-RR targets PSPC1 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen PSPC1 fusion protein Ag10136 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name paraspeckle component 1
Synonyms PSPC 1, PSP1, 242158G7
Calculated Molecular Weight 523 aa, 59 kDa
Observed Molecular Weight 66 kDa
GenBank Accession NumberBC014184
Gene Symbol PSPC1
Gene ID (NCBI) 55269
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ8WXF1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSPC1 (paraspeckle component 1), a known paraspeckle biomarker, is a putative transcription factor that belongs to the Drosophila behavior/human splicing (DBHS) family. PSPC1 was recently identified as a contextual determinant of tumor progression in multiple cancer types involving oncogenic reprogramming to switch proapoptotic TGF-β to prometastatic TGF-β via hijacking of Smad2/3 targeting. Upregulated PSPC1 is correlated with advanced tumor stages and poor survival of patients with breast, lung, and liver cancers. Upregulated PSPC1 potentiates expression of mesenchymal markers, EMT transcription factors (EMT-TF), cancer stem-like cell transcription factors (CSC-TF), and c-Myc-related proliferation genes, thus promoting migration, invasion, spheroid formation, tumor formation, and metastasis.

实验方案

Product Specific Protocols
WB protocol for PSPC1 antibody 84657-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols