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Species Reactivity
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Species Reactivity
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Conjugate
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验证数据展示

  • WB analysis using 84710-3-RR

    WB analysis using 84710-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84710-3-RR (AP4B1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 84710-3-RR

    IP experiment of HeLa using 84710-3-RR

    IP result of anti-AP4B1 (IP:84710-3-RR, 4ug; Detection:84710-3-RR 1:1500) with HeLa cells lysate 1200 ug.

  • Affinity and Kinetic Characterization of 84710-3-RR

    Affinity and Kinetic Characterization of 84710-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84710-3-RR against Human AP4B1 were performed. The affinity constant is 47.1 pM.

AP4B1 Recombinant antibody

AP4B1 Recombinant Antibody for WB, IP, ELISA
Cat No. 84710-3-RR

产品说明书

CloneNo. 242121D4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, ELISA

别名

Beta4-adaptin, Beta subunit of AP-4, AP-4 complex subunit beta-1, AP-4 adaptor complex subunit beta, Adaptor-related protein complex 4 subunit beta-1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMDA-MB-231 cells, MCF-7 cells, HeLa cells, HuH-7 cells, SH-SY5Y cells, NIH/3T3 cells
Positive IP detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84710-3-RR targets AP4B1 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原 AP4B1 fusion protein Ag9348 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 adaptor-related protein complex 4, beta 1 subunit
别名 Beta4-adaptin, Beta subunit of AP-4, AP-4 complex subunit beta-1, AP-4 adaptor complex subunit beta, Adaptor-related protein complex 4 subunit beta-1
计算分子量 739 aa, 83 kDa
观测分子量~75 kDa
GenBank蛋白编号BC014146
基因名称 AP4B1
Gene ID (NCBI) 10717
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9Y6B7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Adaptor protein (AP) complex is a component of intracellular protein transport. The AP4 complex is involved in sorting transmembrane cargo proteins into transport vesicles for trans-Golgi network export. AP4B1 is a member of the AP4 complex, which also includes AP4E1, AP4M1 and AP4S1. Loss-of-function variants in any of these four members can lead to AP-4-associated hereditary spastic paraplegia (HSP), a neurodegenerative disorder (PMID: 30543385). This antibody recognizes AP4B1.

实验方案

Product Specific Protocols
WB protocol for AP4B1 antibody 84710-3-RRDownload protocol
IP protocol for AP4B1 antibody 84710-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols