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×
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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84710-3-RR (same clone as 84710-3-PBS)

    WB analysis using 84710-3-RR (same clone as 84710-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84710-3-RR (AP4B1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84710-3-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 84710-3-RR (same clone as 84710-3-PBS)

    IP experiment of HeLa using 84710-3-RR (same clone as 84710-3-PBS)

    IP result of anti-AP4B1 (IP:84710-3-RR, 4ug; Detection:84710-3-RR 1:1500) with HeLa cells lysate 1200 ug. This data was developed using the same antibody clone with 84710-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84710-3-RR

    Affinity and Kinetic Characterization of 84710-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84710-3-RR against Human AP4B1 were performed. The affinity constant is 47.1 pM.

AP4B1 Recombinant antibody, PBS Only

AP4B1 Uni-rAbTM Recombinant Antibody for WB, IP, Indirect ELISA
Cat No. 84710-3-PBS

产品说明书

CloneNo. 242121D4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, Indirect ELISA

别名

Beta4-adaptin, Beta subunit of AP-4, AP-4 complex subunit beta-1, AP-4 adaptor complex subunit beta, Adaptor-related protein complex 4 subunit beta-1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84710-3-PBS targets AP4B1 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 AP4B1 fusion protein Ag9348 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 adaptor-related protein complex 4, beta 1 subunit
别名 Beta4-adaptin, Beta subunit of AP-4, AP-4 complex subunit beta-1, AP-4 adaptor complex subunit beta, Adaptor-related protein complex 4 subunit beta-1
计算分子量 739 aa, 83 kDa
观测分子量~75 kDa
GenBank蛋白编号BC014146
基因名称 AP4B1
Gene ID (NCBI) 10717
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9Y6B7
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Adaptor protein (AP) complex is a component of intracellular protein transport. The AP4 complex is involved in sorting transmembrane cargo proteins into transport vesicles for trans-Golgi network export. AP4B1 is a member of the AP4 complex, which also includes AP4E1, AP4M1 and AP4S1. Loss-of-function variants in any of these four members can lead to AP-4-associated hereditary spastic paraplegia (HSP), a neurodegenerative disorder (PMID: 30543385). This antibody recognizes AP4B1.