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验证数据展示

  • FC experiment of A549 using 84730-5-RR

    FC experiment of A549 using 84730-5-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug PER1 Recombinant antibody (84730-5-RR, Clone:242096E3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of NIH/3T3 using 84730-5-RR

    IF Staining of NIH/3T3 using 84730-5-RR

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using PER1 antibody (84730-5-RR, Clone: 242096E3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84730-5-RR

    Affinity and Kinetic Characterization of 84730-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84730-5-RR against Human PER1 were performed. The affinity constant is 0.802 nM.

PER1 Recombinant antibody

PER1 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 84730-5-RR

产品说明书

CloneNo. 242096E3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC, FC (Intra), ELISA

别名

period homolog 1 (Drosophila), PER, KIAA0482, hPER1, hPER

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inNIH/3T3 cells
Positive FC (Intra) detected inA549 cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84730-5-RR targets PER1 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 PER1 fusion protein Ag4249 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 period homolog 1 (Drosophila)
别名 period homolog 1 (Drosophila), PER, KIAA0482, hPER1, hPER
计算分子量 1290 aa, 136 kDa
GenBank蛋白编号BC028207
基因名称 PER1
Gene ID (NCBI) 5187
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO15534
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PER1 is a master regulator of circadian rhythm and functions in the nucleus to repress expression of the central circadian clock genes. The periodicity of PER1 abundance, nuclear translocation, and transcriptional repression is regulated by PER1 phosphorylation, ubiquitination, and proteasomal degradation [PMID: 21514639]. It is one component of the circadian clock mechanism which is essential for generating circadian rhythms. It can influences clock function by interacting with other circadian regulatory proteins and transporting them to the nucleus [PMID: 9856465, 20930143].

实验方案

Product Specific Protocols
IF protocol for PER1 antibody 84730-5-RRDownload protocol
FC protocol for PER1 antibody 84730-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols