• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • FC experiment of A549 using 84730-5-RR (same clone as 84730-5-PBS)

    FC experiment of A549 using 84730-5-RR (same clone as 84730-5-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug PER1 Recombinant antibody (84730-5-RR, Clone:242096E3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84730-5-PBS in a different storage buffer formulation.

  • IF Staining of NIH/3T3 using 84730-5-RR (same clone as 84730-5-PBS)

    IF Staining of NIH/3T3 using 84730-5-RR (same clone as 84730-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using PER1 antibody (84730-5-RR, Clone: 242096E3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84730-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84730-5-RR

    Affinity and Kinetic Characterization of 84730-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84730-5-RR against Human PER1 were performed. The affinity constant is 0.802 nM.

PER1 Recombinant antibody, PBS Only

PER1 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84730-5-PBS

产品说明书

CloneNo. 242096E3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC, FC (Intra), Indirect ELISA

别名

period homolog 1 (Drosophila), PER, KIAA0482, hPER1, hPER

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

84730-5-PBS targets PER1 in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 PER1 fusion protein Ag4249 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 period homolog 1 (Drosophila)
别名 period homolog 1 (Drosophila), PER, KIAA0482, hPER1, hPER
计算分子量 1290 aa, 136 kDa
GenBank蛋白编号BC028207
基因名称 PER1
Gene ID (NCBI) 5187
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO15534
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PER1 is a master regulator of circadian rhythm and functions in the nucleus to repress expression of the central circadian clock genes. The periodicity of PER1 abundance, nuclear translocation, and transcriptional repression is regulated by PER1 phosphorylation, ubiquitination, and proteasomal degradation [PMID: 21514639]. It is one component of the circadian clock mechanism which is essential for generating circadian rhythms. It can influences clock function by interacting with other circadian regulatory proteins and transporting them to the nucleus [PMID: 9856465, 20930143].