验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, TF-1 cells, COLO 320 cells, K-562 cells, LNCap cells, Jurkat cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84756-5-RR targets TXNDC5 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | TXNDC5 fusion protein Ag13965 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | thioredoxin domain containing 5 (endoplasmic reticulum) |
| 别名 | Hcc 2, ERp46, ER protein 46, EndoPDI, EC:1.8.4.- |
| 计算分子量 | 432 aa, 48 kDa |
| 观测分子量 | 48 kDa |
| GenBank蛋白编号 | BC001199 |
| 基因名称 | TXNDC5 |
| Gene ID (NCBI) | 81567 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q8NBS9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TXNDC5, or Thioredoxin Domain Containing 5, is a protein-coding gene that plays a significant role in cellular processes, particularly in the endoplasmic reticulum (ER). This gene is essential for the proper functioning of the cellular environment, primarily through its involvement in the formation and rearrangement of disulfide bonds within proteins. Disulfide bonds are crucial for the stability and functionality of many proteins, and TXNDC5 facilitates these processes through its thioredoxin domains, which contain enzymatic activities that promote correct protein folding and maturation in the ER. Recent studies highlight the expression characteristics of TXNDC5 across various diseases. It has been implicated in the promotion of malignant diseases, suggesting a potential role in cancer biology and other pathological conditions. (PMID:25526565; 38629066)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TXNDC5 antibody 84756-5-RR | Download protocol |
| IHC protocol for TXNDC5 antibody 84756-5-RR | Download protocol |
| IF protocol for TXNDC5 antibody 84756-5-RR | Download protocol |
| FC protocol for TXNDC5 antibody 84756-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |