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验证数据展示

  • WB analysis using 84756-5-RR (same clone as 84756-5-PBS)

    WB analysis using 84756-5-RR (same clone as 84756-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84756-5-RR (TXNDC5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84756-5-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84756-5-RR (same clone as 84756-5-PBS)

    IF Staining of HeLa using 84756-5-RR (same clone as 84756-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TXNDC5 antibody (84756-5-RR, Clone: 242195G7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84756-5-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 84756-5-RR (same clone as 84756-5-PBS)

    FC experiment of HeLa using 84756-5-RR (same clone as 84756-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug TXNDC5 Recombinant antibody (84756-5-RR, Clone:242195G7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84756-5-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 84756-5-RR (same clone as 84756-5-PBS)

    IHC staining of human stomach cancer using 84756-5-RR (same clone as 84756-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84756-5-RR (TXNDC5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84756-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TXNDC5 antibody included
  • IHC staining of human stomach cancer using 84756-5-RR (same clone as 84756-5-PBS)

    IHC staining of human stomach cancer using 84756-5-RR (same clone as 84756-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84756-5-RR (TXNDC5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84756-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TXNDC5 antibody included
  • Affinity and Kinetic Characterization of 84756-5-RR

    Affinity and Kinetic Characterization of 84756-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84756-5-RR against Human TXNDC5 were performed. The affinity constant is 2.31 nM.

TXNDC5 Recombinant antibody, PBS Only

TXNDC5 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84756-5-PBS

产品说明书

CloneNo. 242195G7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

Hcc 2, ERp46, ER protein 46, EndoPDI, EC:1.8.4.-

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy TXNDC5 Ready-To-Use IHC Kit. TXNDC5 primary antibody included.

产品信息

84756-5-PBS targets TXNDC5 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 TXNDC5 fusion protein Ag13965 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 thioredoxin domain containing 5 (endoplasmic reticulum)
别名 Hcc 2, ERp46, ER protein 46, EndoPDI, EC:1.8.4.-
计算分子量 432 aa, 48 kDa
观测分子量 48 kDa
GenBank蛋白编号BC001199
基因名称 TXNDC5
Gene ID (NCBI) 81567
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ8NBS9
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TXNDC5, or Thioredoxin Domain Containing 5, is a protein-coding gene that plays a significant role in cellular processes, particularly in the endoplasmic reticulum (ER). This gene is essential for the proper functioning of the cellular environment, primarily through its involvement in the formation and rearrangement of disulfide bonds within proteins. Disulfide bonds are crucial for the stability and functionality of many proteins, and TXNDC5 facilitates these processes through its thioredoxin domains, which contain enzymatic activities that promote correct protein folding and maturation in the ER. Recent studies highlight the expression characteristics of TXNDC5 across various diseases. It has been implicated in the promotion of malignant diseases, suggesting a potential role in cancer biology and other pathological conditions. (PMID:25526565; 38629066)