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验证数据展示

  • WB analysis using 84769-4-RR

    WB analysis using 84769-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84769-4-RR (HMGCL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 84769-4-RR

    FC experiment of HeLa using 84769-4-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug HMGCL Recombinant antibody (84769-4-RR, Clone:242224F9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84769-4-RR

    Affinity and Kinetic Characterization of 84769-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84769-4-RR against Human HMGCL were performed. The affinity constant is 1.13 nM.

HMGCL Recombinant antibody

HMGCL Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84769-4-RR

产品说明书

CloneNo. 242224F9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), ELISA

别名

HMG-CoA lyase, HMG CoA lyase, HL, EC:4.1.3.4, 242224F9

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse liver tissue, rat liver tissue, mouse kidney tissue, rat kidney tissue
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84769-4-RR targets HMGCL in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 HMGCL fusion protein Ag10426 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase
别名 HMG-CoA lyase, HMG CoA lyase, HL, EC:4.1.3.4, 242224F9
计算分子量 325 aa, 34 kDa
观测分子量 32 kDa
GenBank蛋白编号BC010570
基因名称 HMGCL
Gene ID (NCBI) 3155
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP35914
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HMGCL (3-hydroxy-3-methylglutaryl-CoA lyase) is a key enzyme in ketogenesis (ketone body formation). It is a ketogenic enzyme in the liver that catalyzes the formation of acetoacetate from HMG-CoA within the mitochondria. It also plays a prominent role in the catabolism of the amino acid leucine.

实验方案

Product Specific Protocols
WB protocol for HMGCL antibody 84769-4-RRDownload protocol
FC protocol for HMGCL antibody 84769-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols