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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84769-4-RR (same clone as 84769-4-PBS)

    WB analysis using 84769-4-RR (same clone as 84769-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84769-4-RR (HMGCL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84769-4-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 84769-4-RR (same clone as 84769-4-PBS)

    FC experiment of HeLa using 84769-4-RR (same clone as 84769-4-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug HMGCL Recombinant antibody (84769-4-RR, Clone:242224F9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84769-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84769-4-RR

    Affinity and Kinetic Characterization of 84769-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84769-4-RR against Human HMGCL were performed. The affinity constant is 1.13 nM.

HMGCL Recombinant antibody, PBS Only

HMGCL Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 84769-4-PBS

产品说明书

CloneNo. 242224F9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), Indirect ELISA

别名

HMG-CoA lyase, HMG CoA lyase, HL, EC:4.1.3.4, 242224F9

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84769-4-PBS targets HMGCL in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 HMGCL fusion protein Ag10426 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase
别名 HMG-CoA lyase, HMG CoA lyase, HL, EC:4.1.3.4, 242224F9
计算分子量 325 aa, 34 kDa
观测分子量 32 kDa
GenBank蛋白编号BC010570
基因名称 HMGCL
Gene ID (NCBI) 3155
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP35914
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HMGCL (3-hydroxy-3-methylglutaryl-CoA lyase) is a key enzyme in ketogenesis (ketone body formation). It is a ketogenic enzyme in the liver that catalyzes the formation of acetoacetate from HMG-CoA within the mitochondria. It also plays a prominent role in the catabolism of the amino acid leucine.