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cIAP2 Recombinant antibody

cIAP2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

242214E11

Cat no : 84779-4-RR

Print datasheet

Synonyms

BIRC3, Cellular inhibitor of apoptosis 2, C IAP2, BIRC 3, Baculoviral IAP repeat-containing protein 3

验证数据展示

  • WB analysis using 84779-4-RR

    WB analysis using 84779-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84779-4-RR (cIAP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of Raji using 84779-4-RR

    IP experiment of Raji using 84779-4-RR

    IP result of anti-cIAP2 (IP:84779-4-RR, 4ug; Detection:84779-4-RR 1:1000) with Raji cells lysate 1600 ug.

  • FC experiment of Raji using 84779-4-RR

    FC experiment of Raji using 84779-4-RR

    1x10^6 Raji cells were intracellularly stained with 0.25 ug cIAP2 Recombinant antibody (84779-4-RR, Clone:242214E11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive WB detected inDaudi cells, Raji cells
Positive IP detected inRaji cells
Positive FC (Intra) detected inRaji cells
Planning an IHC experiment? We recommend our IHCeasy cIAP2/BIRC3 Ready-To-Use IHC Kit. cIAP2/BIRC3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84779-4-RR targets cIAP2 in WB, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), IP, ELISA Application Description
Tested Reactivity human
Immunogen cIAP2 fusion protein Ag20500 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name baculoviral IAP repeat-containing 3
Synonyms BIRC3, Cellular inhibitor of apoptosis 2, C IAP2, BIRC 3, Baculoviral IAP repeat-containing protein 3
Calculated Molecular Weight 604 aa, 68 kDa
Observed Molecular Weight68 kDa
GenBank Accession NumberBC027485
Gene Symbol cIAP2
Gene ID (NCBI) 330
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ13489
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

cIAP2 also known as BIRC3, is a member of the Inhibitor of Apoptosis Protein (IAP) family. cIAP2 is induced by inflammatory stimuli and plays a critical role in regulating adaptive and innate immune responses, cell death, and the production of inflammatory mediators. Its dysregulation is associated with various diseases, including cancer and neuroinflammatory conditions. Understanding the mechanisms by which cIAP2 functions and interacts with other cellular components is essential for developing targeted therapies.

实验方案

Product Specific Protocols
WB protocol for cIAP2 antibody 84779-4-RRDownload protocol
IP protocol for cIAP2 antibody 84779-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols