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验证数据展示

  • WB analysis using 84798-5-RR

    WB analysis using 84798-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84798-5-RR (ATIC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 84798-5-RR

    FC experiment of HeLa using 84798-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug ATIC Recombinant antibody (84798-5-RR, Clone:242324C8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IHC staining of human colon cancer using 84798-5-RR

    IHC staining of human colon cancer using 84798-5-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84798-5-RR (ATIC antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 84798-5-RR

    IHC staining of human colon cancer using 84798-5-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84798-5-RR (ATIC antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ATIC Recombinant antibody

ATIC Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 84798-5-RR

产品说明书

CloneNo. 242324C8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, FC (Intra), ELISA

别名

AICARFT, AICAR transformylase/inosine monophosphate cyclohydrolase, AICAR formyltransferase, AICAR, 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, HCT 116 cells, A549 cells, MCF-7 cells, COLO 320 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84798-5-RR targets ATIC in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 ATIC fusion protein Ag1052 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase
别名 AICARFT, AICAR transformylase/inosine monophosphate cyclohydrolase, AICAR formyltransferase, AICAR, 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase
计算分子量 65 kDa
观测分子量62 kDa
GenBank蛋白编号BC008879
基因名称 ATIC
Gene ID (NCBI) 471
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP31939
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

5-Aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (ATIC, also known as AICAR) is an enzyme that can catalyze the last two reactions in the de novo purine biosynthetic pathway. It has been previously reported to be upregulated and to participate in myeloma and hepatocellular carcinoma progression (PMID: 35251351).

实验方案

Product Specific Protocols
WB protocol for ATIC antibody 84798-5-RRDownload protocol
IHC protocol for ATIC antibody 84798-5-RRDownload protocol
FC protocol for ATIC antibody 84798-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols