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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84798-5-RR (same clone as 84798-5-PBS)

    WB analysis using 84798-5-RR (same clone as 84798-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84798-5-RR (ATIC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84798-5-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 84798-5-RR (same clone as 84798-5-PBS)

    IHC staining of human colon cancer using 84798-5-RR (same clone as 84798-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84798-5-RR (ATIC antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84798-5-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 84798-5-RR (same clone as 84798-5-PBS)

    FC experiment of HeLa using 84798-5-RR (same clone as 84798-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug ATIC Recombinant antibody (84798-5-RR, Clone:242324C8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84798-5-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 84798-5-RR (same clone as 84798-5-PBS)

    IHC staining of human colon cancer using 84798-5-RR (same clone as 84798-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84798-5-RR (ATIC antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84798-5-PBS in a different storage buffer formulation.

ATIC Recombinant antibody, PBS Only

ATIC Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 84798-5-PBS

产品说明书

CloneNo. 242324C8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, FC (Intra), Indirect ELISA

别名

AICARFT, AICAR transformylase/inosine monophosphate cyclohydrolase, AICAR formyltransferase, AICAR, 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84798-5-PBS targets ATIC in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 ATIC fusion protein Ag1052 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase
别名 AICARFT, AICAR transformylase/inosine monophosphate cyclohydrolase, AICAR formyltransferase, AICAR, 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase
计算分子量 65 kDa
观测分子量62 kDa
GenBank蛋白编号BC008879
基因名称 ATIC
Gene ID (NCBI) 471
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP31939
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

5-Aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (ATIC, also known as AICAR) is an enzyme that can catalyze the last two reactions in the de novo purine biosynthetic pathway. It has been previously reported to be upregulated and to participate in myeloma and hepatocellular carcinoma progression (PMID: 35251351).