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验证数据展示

  • IF Staining of HepG2 using 84855-1-RR (same clone as 84855-1-PBS)

    IF Staining of HepG2 using 84855-1-RR (same clone as 84855-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FOXP4 antibody (84855-1-RR, Clone: 242356B4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84855-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84855-1-RR (same clone as 84855-1-PBS)

    IF Staining of HeLa using 84855-1-RR (same clone as 84855-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FOXP4 antibody (84855-1-RR, Clone: 242356B4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84855-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 84855-1-RR (same clone as 84855-1-PBS)

    FC experiment of HepG2 using 84855-1-RR (same clone as 84855-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug FOXP4 Recombinant antibody (84855-1-RR, Clone:242356B4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84855-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01621-2

    Sandwich ELISA standard curve of MP01621-2

    Sandwich ELISA standard curve of MP01621-2, Human FOXP4 Recombinant Matched Antibody Pair - PBS only. 84855-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag10047. 84855-3-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

  • Sample test of MP01621-2

    Sample test of MP01621-2

    The mean FOXP4 concentration was determined to be 5.4 ng/mL in HepG2 cell extract based on a 1.0 mg/mL extract load.

  • Cytometric bead array standard curve of MP01621-1

    Cytometric bead array standard curve of MP01621-1

    Cytometric bead array standard curve of MP01621-1, FOXP4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84855-1-PBS. Detection antibody: 84855-2-PBS. Standard: Ag10047. Range: 1.563-100 ng/mL.

  • Affinity and Kinetic Characterization of 84855-1-RR

    Affinity and Kinetic Characterization of 84855-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84855-1-RR against Human FOXP4 were performed. The affinity constant is 0.402 nM.

FOXP4 Recombinant antibody, PBS Only (Capture)

FOXP4 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84855-1-PBS

产品说明书

CloneNo. 242356B4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

Fork head-related protein-like A, FKHLA, 242356B4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy FOXP4 Ready-To-Use IHC Kit. FOXP4 primary antibody included.

产品信息

84855-1-PBS targets FOXP4 as part of a matched antibody pair:

MP01621-1: 84855-1-PBS capture and 84855-2-PBS detection (validated in Cytometric bead array)

MP01621-2: 84855-1-PBS capture and 84855-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 FOXP4 fusion protein Ag10047 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 forkhead box P4
别名 Fork head-related protein-like A, FKHLA, 242356B4
计算分子量 678 aa, 73 kDa
GenBank蛋白编号BC052803
基因名称 FOXP4
Gene ID (NCBI) 116113
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8IVH2
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FOXP4, also named as FKHLA, belongs to the FOX family. FOXP4 has been implicated in diverse biological processes in tumor initiation and progression. It has been demonstrated that FOXP4 is significantly upregulated in breast cancer, hepatocellular carcinoma and oral squamous cell carcinoma (PMID: 34590150). FOXP4 has 3 isoforms with the molecular mass of 72-73 kDa.