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验证数据展示

  • WB analysis using 84876-5-RR

    WB analysis using 84876-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84876-5-RR (NNMT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 84876-5-RR

    FC experiment of HepG2 using 84876-5-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug NNMT Recombinant antibody (84876-5-RR, Clone:242394B7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IP experiment of L02 using 84876-5-RR

    IP experiment of L02 using 84876-5-RR

    IP result of anti-NNMT (IP:84876-5-RR, 4ug; Detection:84876-5-RR 1:1500) with L02 cells lysate 1120 ug.

  • Affinity and Kinetic Characterization of 84876-5-RR

    Affinity and Kinetic Characterization of 84876-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84876-5-RR against Human NNMT were performed. The affinity constant is 0.824 nM.

NNMT Recombinant antibody

NNMT Uni-rAbTM Recombinant Antibody for WB, FC (Intra), IP, ELISA
Cat No. 84876-5-RR

产品说明书

CloneNo. 242394B7

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, FC (Intra), IP, ELISA

别名

Nicotinamide N-methyltransferase, EC:2.1.1.1, 242394B7

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inL02 cells, SGC-7901 cells, rat liver tissue
Positive IP detected inL02 cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84876-5-RR targets NNMT in WB, FC (Intra), IP, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, FC (Intra), IP, ELISA Application Description
经测试反应性 human, rat
免疫原 NNMT fusion protein Ag7197 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 nicotinamide N-methyltransferase
别名 Nicotinamide N-methyltransferase, EC:2.1.1.1, 242394B7
计算分子量 30 kDa
观测分子量 30 kDa
GenBank蛋白编号BC000234
基因名称 NNMT
Gene ID (NCBI) 4837
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP40261
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NNMT can catalyze the N-methylation of nicotinamide using the universal methyl donor S-adenosyl-L-methionine to form N1-methylnicotinamide and S-adenosyl-L-homocysteine, a predominant nicotinamide/vitamin B3 clearance pathway (PMID: 21823666; 23455543; 8182091).

实验方案

Product Specific Protocols
WB protocol for NNMT antibody 84876-5-RRDownload protocol
IP protocol for NNMT antibody 84876-5-RRDownload protocol
FC protocol for NNMT antibody 84876-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols