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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
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Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84876-5-RR (same clone as 84876-5-PBS)

    WB analysis using 84876-5-RR (same clone as 84876-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84876-5-RR (NNMT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84876-5-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 84876-5-RR (same clone as 84876-5-PBS)

    FC experiment of HepG2 using 84876-5-RR (same clone as 84876-5-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug NNMT Recombinant antibody (84876-5-RR, Clone:242394B7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84876-5-PBS in a different storage buffer formulation.

  • IP experiment of L02 using 84876-5-RR (same clone as 84876-5-PBS)

    IP experiment of L02 using 84876-5-RR (same clone as 84876-5-PBS)

    IP result of anti-NNMT (IP:84876-5-RR, 4ug; Detection:84876-5-RR 1:1500) with L02 cells lysate 1120 ug. This data was developed using the same antibody clone with 84876-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84876-5-RR

    Affinity and Kinetic Characterization of 84876-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84876-5-RR against Human NNMT were performed. The affinity constant is 0.824 nM.

NNMT Recombinant antibody, PBS Only

NNMT Uni-rAbTM Recombinant Antibody for WB, FC (Intra), IP, Indirect ELISA
Cat No. 84876-5-PBS

产品说明书

CloneNo. 242394B7

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, FC (Intra), IP, Indirect ELISA

别名

Nicotinamide N-methyltransferase, EC:2.1.1.1, 242394B7

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84876-5-PBS targets NNMT in WB, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原 NNMT fusion protein Ag7197 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 nicotinamide N-methyltransferase
别名 Nicotinamide N-methyltransferase, EC:2.1.1.1, 242394B7
计算分子量 30 kDa
观测分子量 30 kDa
GenBank蛋白编号BC000234
基因名称 NNMT
Gene ID (NCBI) 4837
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP40261
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NNMT can catalyze the N-methylation of nicotinamide using the universal methyl donor S-adenosyl-L-methionine to form N1-methylnicotinamide and S-adenosyl-L-homocysteine, a predominant nicotinamide/vitamin B3 clearance pathway (PMID: 21823666; 23455543; 8182091).