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验证数据展示

  • WB analysis using 84967-1-RR (same clone as 84967-1-PBS)

    WB analysis using 84967-1-RR (same clone as 84967-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84967-1-RR (MEK1/2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84967-1-PBS in a different storage buffer formulation.

  • FC experiment of Jurkat using 84967-1-RR (same clone as 84967-1-PBS)

    FC experiment of Jurkat using 84967-1-RR (same clone as 84967-1-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug MEK1/2 Recombinant Antibody (84967-1-RR, Clone:242614G3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84967-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01692-1

    Sandwich ELISA standard curve of MP01692-1

    Sandwich ELISA standard curve of MP01692-1, Human MEK2 Recombinant Matched Antibody Pair - PBS only. 84967-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1481. 84967-1-PBS was HRP conjugated as the detection antibody. Range: 0.781-50 ng/mL.

  • Sample test of MP01692-1

    Sample test of MP01692-1

    The mean MEK2 concentration was determined to be 45.61 ng/mL in A549 cell extract based on a 1.50 mg/mL extract load and 44.07 ng/mL in HeLa cell extract based on a 1.00 mg/mL extract load.

  • IF Staining of Jurkat using 84967-1-RR (same clone as 84967-1-PBS)

    IF Staining of Jurkat using 84967-1-RR (same clone as 84967-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using MEK1/2 antibody (84967-1-RR, Clone: 242614G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84967-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01692-1

    Cytometric bead array standard curve of MP01692-1

    Cytometric bead array standard curve of MP01692-1, MEK2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84967-2-PBS. Detection antibody: 84967-1-PBS. Standard: Ag1481. Range: 0.625-80 ng/mL.

  • Cytometric bead array standard curve of MP01692-2

    Cytometric bead array standard curve of MP01692-2

    Cytometric bead array standard curve of MP01692-2, MEK2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84967-1-PBS. Detection antibody: 84967-3-PBS. Standard: Ag1481. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 84967-1-RR

    Affinity and Kinetic Characterization of 84967-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84967-1-RR against Human MEK1/2 were performed. The affinity constant is below 1 pM.

MEK1/2 Recombinant antibody, PBS Only (Capture/Detector)

MEK1/2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84967-1-PBS

产品说明书

CloneNo. 242614G3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

MAP2K2, MEK2, MAP kinase kinase 2, ERK activator kinase 2, EC:2.7.12.2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy MAP2K2 Ready-To-Use IHC Kit. MAP2K2 primary antibody included.

产品信息

84967-1-PBS targets MEK1/2 as part of a matched antibody pair:

MP01692-1: 84967-2-PBS capture and 84967-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP01692-2: 84967-1-PBS capture and 84967-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse, rat
免疫原 MEK1/2 fusion protein Ag1481 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mitogen-activated protein kinase kinase 2
别名 MAP2K2, MEK2, MAP kinase kinase 2, ERK activator kinase 2, EC:2.7.12.2
计算分子量 44 kDa
观测分子量 44 kDa
GenBank蛋白编号BC018645
基因名称 MEK2
Gene ID (NCBI) 5605
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP36507
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MEK2(MAPK/ERK kinase 2) is also named as MAP2K2, MKK2, PRKMK2 and belongs to the MAP kinase kinase subfamily. MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). MEK1 and MEK2 are closely related, dual-specificity tyrosine/threonine protein kinases found in the Ras/Raf/MEK/ERK mitogen-activated protein kinase (MAPK) signaling pathway. 84967-1-RR can recognize both the MEK2 and MEK1.