验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, A431 cells, mouse skin tissue, rat skin tissue |
| Positive IHC detected in | human skin cancer tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, A431 cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:3000-1:12000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84978-1-RR targets Cytokeratin 17-specific in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | keratin 17 |
| 别名 | Cytokeratin 17, KRT17, 242240A8, CK 17, CK-17 |
| 计算分子量 | 48 kDa |
| 观测分子量 | 48 kDa |
| GenBank蛋白编号 | NM_000422 |
| 基因名称 | Cytokeratin 17 |
| Gene ID (NCBI) | 3872 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q04695 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Keratins are a large family of proteins that form the intermediate filaments that make up the cytoskeleton. Type I keratins are a group of acidic intermediate filament proteins and type II keratins are the basic or neutral counterparts. Keratin 17 is encoded by the KRT17 gene and is a type I cytokeratin found in nail beds, hair follicles, sebaceous glands, and epidermal appendages.The molecular weight of cytokeratin 17 is approximately 49 kDa.Cytokeratin 17 plays a role in the formation and maintenance of epidermal appendages, especially in determining the shape and orientation of hair. It is required for the maintenance of the anagen (growth) state of hair follicles by modulating the function of TNF-alpha for hair cycling and is also involved in tissue repair (PMID: 16702408).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Cytokeratin 17-specific antibody 84978-1-RR | Download protocol |
| IHC protocol for Cytokeratin 17-specific antibody 84978-1-RR | Download protocol |
| IF protocol for Cytokeratin 17-specific antibody 84978-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
