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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 85028-1-RR (same clone as 85028-1-PBS)

    WB analysis using 85028-1-RR (same clone as 85028-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85028-1-RR (HEXIM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85028-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 85028-1-RR (same clone as 85028-1-PBS)

    IF Staining of HeLa using 85028-1-RR (same clone as 85028-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HEXIM1 antibody (85028-1-RR, Clone: 242587A2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85028-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01740-2

    Cytometric bead array standard curve of MP01740-2

    Cytometric bead array standard curve of MP01740-2, HEXIM1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85028-3-PBS. Detection antibody: 85028-1-PBS. Standard: Ag8144. Range: 0.156-20 ng/mL.

HEXIM1 Recombinant antibody, PBS Only (Detector)

HEXIM1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 85028-1-PBS
CloneNo. 242587A2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

Hexamethylene bis-acetamide-inducible protein 1, Estrogen down-regulated gene 1 protein, EDG1, CLP1, CLP 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy HEXIM1 Ready-To-Use IHC Kit. HEXIM1 primary antibody included.

产品信息

85028-1-PBS targets HEXIM1 as part of a matched antibody pair:

MP01740-2: 85028-3-PBS capture and 85028-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen HEXIM1 fusion protein Ag8144 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name hexamethylene bis-acetamide inducible 1
Synonyms Hexamethylene bis-acetamide-inducible protein 1, Estrogen down-regulated gene 1 protein, EDG1, CLP1, CLP 1
Calculated Molecular Weight 41 kDa
Observed Molecular Weight65 kDa
GenBank Accession NumberBC006460
Gene Symbol HEXIM1
Gene ID (NCBI) 10614
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO94992
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HEXIM1, also named CLP1, EDG1, HIS1 or MAQ1, is best known as the inhibitor of positive transcription elongation factor b, which regulates the transcription elongation of RNA polymerase II and controls 60-70% of mRNA synthesis. HEXIM1 regulates NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity. Previous studies showed that HEXIM1 interacts with two key p53 regulators, nucleophosmin and HDM2, implying a possible connection between HEXIM1 and the p53 signaling pathway.The mainstream MW of this protein is 65-70 kDa and 54 kDa ( PMID: 33627647, PMID: 20976203, PMID: 28254838).