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验证数据展示

  • WB analysis using 85028-1-RR

    WB analysis using 85028-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85028-1-RR (HEXIM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 85028-1-RR

    IF Staining of HeLa using 85028-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HEXIM1 antibody (85028-1-RR, Clone: 242587A2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HeLa using 85028-1-RR

    FC experiment of HeLa using 85028-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug HEXIM1 Recombinant antibody (85028-1-RR, Clone:242587A2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

HEXIM1 Recombinant antibody

HEXIM1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 85028-1-RR

产品说明书

CloneNo. 242587A2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

242587A2, CLP 1, CLP1, EDG1, Estrogen down-regulated gene 1 protein

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells, A431 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy HEXIM1 Ready-To-Use IHC Kit. HEXIM1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85028-1-RR targets HEXIM1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 HEXIM1 fusion protein Ag8144 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 hexamethylene bis-acetamide inducible 1
别名 242587A2, CLP 1, CLP1, EDG1, Estrogen down-regulated gene 1 protein
计算分子量 41 kDa
观测分子量65 kDa
GenBank蛋白编号BC006460
基因名称 HEXIM1
Gene ID (NCBI) 10614
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO94992
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HEXIM1, also named CLP1, EDG1, HIS1 or MAQ1, is best known as the inhibitor of positive transcription elongation factor b, which regulates the transcription elongation of RNA polymerase II and controls 60-70% of mRNA synthesis. HEXIM1 regulates NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity. Previous studies showed that HEXIM1 interacts with two key p53 regulators, nucleophosmin and HDM2, implying a possible connection between HEXIM1 and the p53 signaling pathway.The mainstream MW of this protein is 65-70 kDa and 54 kDa ( PMID: 33627647, PMID: 20976203, PMID: 28254838).

实验方案

Product Specific Protocols
WB protocol for HEXIM1 antibody 85028-1-RRDownload protocol
IF protocol for HEXIM1 antibody 85028-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols