• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 85028-1-RR (same clone as 85028-1-PBS)

    WB analysis using 85028-1-RR (same clone as 85028-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85028-1-RR (HEXIM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85028-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 85028-1-RR (same clone as 85028-1-PBS)

    IF Staining of HeLa using 85028-1-RR (same clone as 85028-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HEXIM1 antibody (85028-1-RR, Clone: 242587A2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85028-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 85028-1-RR (same clone as 85028-1-PBS)

    FC experiment of HeLa using 85028-1-RR (same clone as 85028-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug HEXIM1 Recombinant antibody (85028-1-RR, Clone:242587A2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 85028-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01740-2

    Cytometric bead array standard curve of MP01740-2

    Cytometric bead array standard curve of MP01740-2, HEXIM1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85028-3-PBS. Detection antibody: 85028-1-PBS. Standard: Ag8144. Range: 0.156-20 ng/mL.

HEXIM1 Recombinant antibody, PBS Only (Detector)

HEXIM1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 85028-1-PBS

产品说明书

CloneNo. 242587A2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

242587A2, CLP 1, CLP1, EDG1, Estrogen down-regulated gene 1 protein

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy HEXIM1 Ready-To-Use IHC Kit. HEXIM1 primary antibody included.

产品信息

85028-1-PBS targets HEXIM1 as part of a matched antibody pair:

MP01740-2: 85028-3-PBS capture and 85028-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 HEXIM1 fusion protein Ag8144 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 hexamethylene bis-acetamide inducible 1
别名 242587A2, CLP 1, CLP1, EDG1, Estrogen down-regulated gene 1 protein
计算分子量 41 kDa
观测分子量65 kDa
GenBank蛋白编号BC006460
基因名称 HEXIM1
Gene ID (NCBI) 10614
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO94992
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HEXIM1, also named CLP1, EDG1, HIS1 or MAQ1, is best known as the inhibitor of positive transcription elongation factor b, which regulates the transcription elongation of RNA polymerase II and controls 60-70% of mRNA synthesis. HEXIM1 regulates NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity. Previous studies showed that HEXIM1 interacts with two key p53 regulators, nucleophosmin and HDM2, implying a possible connection between HEXIM1 and the p53 signaling pathway.The mainstream MW of this protein is 65-70 kDa and 54 kDa ( PMID: 33627647, PMID: 20976203, PMID: 28254838).