• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • Sandwich ELISA standard curve of MP01960-1

    Sandwich ELISA standard curve of MP01960-1

    Sandwich ELISA standard curve of MP01960-1, Human Filamin B Recombinant Matched Antibody Pair - PBS only. 85202-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard SY00901. 85202-1-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

  • Sample test of MP01960-1

    Sample test of MP01960-1

    The mean Filamin B concentration was determined to be 4.9 ng/mL in A549 cell extract based on a 2.6 mg/mL extract load and 5.5 ng/mL in HeLa cell extract based on a 1.5 mg/mL extract load.

  • Sample test of MP01960-1

    Sample test of MP01960-1

    Serum of six individual healthy human donors was measured. The Filamin B concentration of detected samples was determined to be 1.1 ng/mL with a range of 0.4-1.9 ng/mL.

  • IF Staining of HeLa using 85202-1-RR (same clone as 85202-1-PBS)

    IF Staining of HeLa using 85202-1-RR (same clone as 85202-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Filamin B antibody (85202-1-RR, Clone: 242275B7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85202-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 85202-1-RR (same clone as 85202-1-PBS)

    IF Staining of A549 using 85202-1-RR (same clone as 85202-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using Filamin B antibody (85202-1-RR, Clone: 242275B7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85202-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293T using 85202-1-RR (same clone as 85202-1-PBS)

    FC experiment of HEK-293T using 85202-1-RR (same clone as 85202-1-PBS)

    1x10^6 HEK-293T cells were intracellularly stained with 0.25 ug Filamin B Recombinant antibody (85202-1-RR, Clone:242275B7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 85202-1-PBS in a different storage buffer formulation.

Filamin B Recombinant antibody, PBS Only (Detector)

Filamin B Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 85202-1-PBS

产品说明书

CloneNo. 242275B7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

别名

FLNB, Filamin-3, Beta-filamin, Actin-binding-like protein, ABP-280 homolog

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

85202-1-PBS targets Filamin B as part of a matched antibody pair:

MP01960-1: 85202-2-PBS capture and 85202-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 filamin B, beta (actin binding protein 278)
别名 FLNB, Filamin-3, Beta-filamin, Actin-binding-like protein, ABP-280 homolog
计算分子量 278 kDa
GenBank蛋白编号NM_001457
基因名称 FLNB
Gene ID (NCBI) 2317
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO75369
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FLNB, also named as FLN1L, FLN3, TABP, TAP and Fh1, belongs to the filamin family. FLNB connects cell membrane constituents to the actin cytoskeleton. It may promote orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. It is an anchors various transmembrane proteins to the actin cytoskeleton. Interaction with FLNA, FLNB may allow neuroblast migration from the ventricular zone into the cortical plate. Various interactions and localizations of isoforms affect myotube morphology and myogenesis.