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Anti-Mouse MCP-1/CCL2 Rabbit Recombinant Antibody

MCP-1/CCL2 Uni-rAbTM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

230483A9

Cat no : 98028-1-RR

Print datasheet

Synonyms

Ccl2, MCP1, MCP-1, chemokine (C C motif) ligand 2, CCL-2

验证数据展示

  • FC experiment of RAW 264.7 using 98028-1-RR

    FC experiment of RAW 264.7 using 98028-1-RR

    1x10^6 unstimulated or LPS and Brefeldin A treated RAW 264.7 cells were intracellularly stained with 0.25 ug Anti-Mouse MCP-1 Rabbit Recombinant Antibody (98028-1-RR, Clone: 230483A9) and PE-conjugated Goat Anti-Rabbit IgG. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of RAW 264.7 using 98028-1-RR

    FC experiment of RAW 264.7 using 98028-1-RR

    1x10^6 unstimulated (black) or LPS and Brefeldin A treated RAW 264.7 cells were intracellularly stained with 0.25 ug Anti-Mouse MCP-1 Rabbit Recombinant Antibody (98028-1-RR, Clone: 230483A9) (red) or 0.25 ug Isotype Control (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 98028-1-RR

    Affinity and Kinetic Characterization of 98028-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98028-1 -RR against Mouse Mcp1 were performed. The affinity constant is below 1 nM.

经过测试的应用

Positive FC (Intra) detected inLPS and Brefeldin A treated RAW 264.7 cells

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 98028-1-RR is MCP-1/CCL2 Fusion Protein expressed in E. coli.

Tested Applications FC (Intra) Application Description
Tested Reactivity mouse
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name chemokine (C-C motif) ligand 2
Synonyms Ccl2, MCP1, MCP-1, chemokine (C C motif) ligand 2, CCL-2
GenBank Accession NumberNM-011333
Gene Symbol MCP-1/CCL2
Gene ID (NCBI) 20296
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP10148
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

Monocyte chemotactic protein 1 (MCP-1; also known as CCL2), is chemotactic for monocyte/macrophage, B-cell, and T-lymphocyte, and belongs to the CC subfamily of chemokines. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The human ortholog has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis.

实验方案

Product Specific Protocols
FC protocol for MCP-1/CCL2 antibody 98028-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols