验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
| Positive FC detected in | PMA treated U-937 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
98048-1-RR targets uPAR/CD87 in IF/ICC, FC applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC, FC Application Description |
| 经测试反应性 | human |
| 免疫原 | Recombinant protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | PLAUR |
| 别名 | PLAUR, UPAR, U PAR, MO3, CD87 |
| 计算分子量 | 37 kDa |
| GenBank蛋白编号 | NM_002659.4 |
| 基因名称 | uPAR |
| Gene ID (NCBI) | 5329 |
| RRID | AB_3672195 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q03405-1 |
| 储存缓冲液 | PBS with 0.09% sodium azide. |
| 储存条件 | Store at 2-8°C. Stable for one year after shipment. |
背景介绍
uPAR, also known as CD87, is a highly glycosylated, GPI-anchored membrane protein. In addition to the membrane-anchored form, uPAR is released from the plasma membrane by cleavage of the GPI anchor and can be found as a soluble form (suPAR). uPAR contains three homologous domains (D1-D3) of which the N-terminal one (D1) represents the uPA-binding domain. After binding to uPAR, uPA cleaves plasminogen, generating the active protease plasmin which is involved in a wide variety of physiologic and pathologic processes. In addition to regulating proteolysis, uPAR has important function in cell adhesion, migration and proliferation. Studies reveal that uPAR expression is elevated during inflammation and tissue remodeling and in many human cancers, in which it frequently indicates poor prognosis. (PMID 20027185; 12461559)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for uPAR/CD87 antibody 98048-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |