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验证数据展示

  • FC experiment of human peripheral blood leukocytes using 98084-1-RR

    FC experiment of human peripheral blood leukocytes using 98084-1-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human SELPLG/CD162 Rabbit Recombinant Antibody (98084-1-RR, Clone:241297F10), or Isotype Control and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed.

  • FC experiment of human peripheral blood leukocytes using 98084-1-RR

    FC experiment of human peripheral blood leukocytes using 98084-1-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human SELPLG/CD162 Rabbit Recombinant Antibody (98084-1-RR, Clone:241297F10) (red), or Isotype Control (blue) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed.

  • Affinity and Kinetic Characterization of 98084-1-RR

    Affinity and Kinetic Characterization of 98084-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98084-1-RR against Human SELPLG/CD162 were performed. The affinity constant is 0.699 nM.

Anti-Human SELPLG/CD162 Rabbit Recombinant Antibody

SELPLG/CD162 Uni-rAbTM Recombinant Antibody for FC
Cat No. 98084-1-RR

产品说明书

CloneNo. 241297F10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

SELPLG, PSGL1, PSGL 1, P-selectin glycoprotein ligand 1, CLA

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC detected inhuman peripheral blood leukocytes

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98084-1-RR targets SELPLG/CD162 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Recombinant Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 selectin P ligand
别名 SELPLG, PSGL1, PSGL 1, P-selectin glycoprotein ligand 1, CLA
计算分子量 412 aa, 43 kDa
GenBank蛋白编号BC029782
基因名称 SELPLG
Gene ID (NCBI) 6404
RRIDAB_3672231
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ14242
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

SELPLG (selectin P ligand), also known as CD162 or PSGL-1, is a 120 kDa type I transmembrane mucin-like glycoprotein. It is an adhesion molecule expressed on the surface of neutrophils, monocytes, and most lymphocytes as a homodimer of approximately 230-250 kDa (PMID: 7545173; 1378449; 9353350). SELPLG plays a critical role in the early stages of inflammation due to its ability to bind E-selectin (CD62E), L-selectin (CD62L), and P-selectin (CD62P) (PMID: 35597982).

实验方案

Product Specific Protocols
FC protocol for SELPLG/CD162 antibody 98084-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols