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验证数据展示

  • FC experiment of human peripheral blood leukocytes using 98084-1-RR (same clone as 98084-1-PBS)

    FC experiment of human peripheral blood leukocytes using 98084-1-RR (same clone as 98084-1-PBS)

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human SELPLG/CD162 Rabbit Recombinant Antibody (98084-1-RR, Clone:241297F10), or Isotype Control and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed. This data was developed using the same antibody clone with 98084-1-PBS in a different storage buffer formulation.

  • FC experiment of human peripheral blood leukocytes using 98084-1-RR (same clone as 98084-1-PBS)

    FC experiment of human peripheral blood leukocytes using 98084-1-RR (same clone as 98084-1-PBS)

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human SELPLG/CD162 Rabbit Recombinant Antibody (98084-1-RR, Clone:241297F10) (red), or Isotype Control (blue) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed. This data was developed using the same antibody clone with 98084-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98084-1-RR

    Affinity and Kinetic Characterization of 98084-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98084-1-RR against Human SELPLG/CD162 were performed. The affinity constant is 0.699 nM.

Anti-Human SELPLG/CD162 Rabbit Recombinant Antibody, PBS Only

SELPLG/CD162 Uni-rAbTM Recombinant Antibody for FC
Cat No. 98084-1-PBS

产品说明书

CloneNo. 241297F10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

SELPLG, PSGL1, PSGL 1, P-selectin glycoprotein ligand 1, CLA

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

98084-1-PBS targets SELPLG/CD162 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Recombinant Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 selectin P ligand
别名 SELPLG, PSGL1, PSGL 1, P-selectin glycoprotein ligand 1, CLA
计算分子量 412 aa, 43 kDa
GenBank蛋白编号BC029782
基因名称 SELPLG
Gene ID (NCBI) 6404
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ14242
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SELPLG (selectin P ligand), also known as CD162 or PSGL-1, is a 120 kDa type I transmembrane mucin-like glycoprotein. It is an adhesion molecule expressed on the surface of neutrophils, monocytes, and most lymphocytes as a homodimer of approximately 230-250 kDa (PMID: 7545173; 1378449; 9353350). SELPLG plays a critical role in the early stages of inflammation due to its ability to bind E-selectin (CD62E), L-selectin (CD62L), and P-selectin (CD62P) (PMID: 35597982).