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Anti-Human SELPLG/CD162 Rabbit Recombinant Antibody

SELPLG/CD162 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241297F10

Cat no : 98084-1-RR

Print datasheet

Synonyms

SELPLG, PSGL1, PSGL 1, P-selectin glycoprotein ligand 1, CLA

验证数据展示

  • FC experiment of human peripheral blood leukocytes using 98084-1-RR

    FC experiment of human peripheral blood leukocytes using 98084-1-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human SELPLG/CD162 Rabbit Recombinant Antibody (98084-1-RR, Clone:241297F10), or Isotype Control and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed.

  • FC experiment of human peripheral blood leukocytes using 98084-1-RR

    FC experiment of human peripheral blood leukocytes using 98084-1-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human SELPLG/CD162 Rabbit Recombinant Antibody (98084-1-RR, Clone:241297F10) (red), or Isotype Control (blue) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed.

  • Affinity and Kinetic Characterization of 98084-1-RR

    Affinity and Kinetic Characterization of 98084-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98084-1-RR against Human SELPLG/CD162 were performed. The affinity constant is 0.699 nM.

经过测试的应用

Positive FC detected inhuman peripheral blood leukocytes

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98084-1-RR targets SELPLG/CD162 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Recombinant Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name selectin P ligand
Synonyms SELPLG, PSGL1, PSGL 1, P-selectin glycoprotein ligand 1, CLA
Calculated Molecular Weight 412 aa, 43 kDa
GenBank Accession NumberBC029782
Gene Symbol SELPLG
Gene ID (NCBI) 6404
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ14242
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

背景介绍

SELPLG (selectin P ligand), also known as CD162 or PSGL-1, is a 120 kDa type I transmembrane mucin-like glycoprotein. It is an adhesion molecule expressed on the surface of neutrophils, monocytes, and most lymphocytes as a homodimer of approximately 230-250 kDa (PMID: 7545173; 1378449; 9353350). SELPLG plays a critical role in the early stages of inflammation due to its ability to bind E-selectin (CD62E), L-selectin (CD62L), and P-selectin (CD62P) (PMID: 35597982).

实验方案

Product Specific Protocols
FC protocol for SELPLG/CD162 antibody 98084-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols