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Anti-Human MICA/MICB Rabbit Recombinant Antibody

MICA/MICB Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

240735D5

Cat no : 98110-1-RR

Print datasheet

Synonyms

MICB, MICA, PERB11.1, MIC A, 240735D5

验证数据展示

  • FC experiment of HeLa using 98110-1-RR

    FC experiment of HeLa using 98110-1-RR

    1x10^6 HeLa cells (left) or Jurkat cells (right) were surface stained with 0.25 ug Anti-Human MICA/MICB Rabbit Recombinant Antibody (98110-1-RR, Clone: 240735D5) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Isotype Control (blue). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98110-1-RR

    Affinity and Kinetic Characterization of 98110-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98110-1-RR against Human MICA were performed. The affinity constant is 0.907 nM.

经过测试的应用

Positive FC detected inHeLa cells

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98110-1-RR targets MICA/MICB in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name MHC class I polypeptide-related sequence A
Synonyms MICB, MICA, PERB11.1, MIC A, 240735D5
Calculated Molecular Weight43 kDa
GenBank Accession NumberAAH16929.1
Gene Symbol MICA
Gene ID (NCBI) 4276
RRIDAB_3672257
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

背景介绍

Human MHC class I chain-related genes located within the HLA class I region of chromosome 6 encode MHC class I chain-related A and B (MICA and MICB) (PMID: 11429322). MICA and MICB are stress-inducible surface molecules that are not associated with β2-microglobulin and do not present peptides (PMID: 9497295). They are expressed in intestinal epithelium and many epithelial tumors (PMID: 10359807). MICA and MICB are ligands for NKG2D which is an activating receptor that is expressed on most natural killer (NK) cells, CD8 αβ T cells, and γδ T cells (PMID: 10426993; 11491531). This antibody recognizes both MICA and MICB.

实验方案

Product Specific Protocols
FC protocol for MICA/MICB antibody 98110-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols