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Anti-Human Ki-67 Rabbit Recombinant Antibody

Ki-67 Uni-rAbTM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

241499B1

Cat no : 98143-2-RR

Print datasheet

Synonyms

MKI67, KI67, Ki 67, Antigen KI-67, Antigen identified by monoclonal antibody Ki-67

验证数据展示

  • FC experiment of human PBMCs using 98143-2-RR

    FC experiment of human PBMCs using 98143-2-RR

    1x10^6 untreated or PHA-treated human PBMCs were intracellularly stained with 0.25 ug Ki-67 Recombinant Antibody (98143-2-RR, Clone: 241499B1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD3. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • FC experiment of human PBMCs using 98143-2-RR

    FC experiment of human PBMCs using 98143-2-RR

    1x10^6 PHA treated human PBMCs were intracellularly stained with 0.25 ug Ki-67 Recombinant Antibody (98143-2-RR, Clone: 241499B1) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were intracellularly stained with 0.25 ug Ki-67 Recombinant Antibody (98143-2-RR, Clone:241499B1) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC (Intra) detected inPHA treated human PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98143-2-RR targets Ki-67 in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name antigen identified by monoclonal antibody Ki-67
Synonyms MKI67, KI67, Ki 67, Antigen KI-67, Antigen identified by monoclonal antibody Ki-67
Calculated Molecular Weight 359 kDa
GenBank Accession NumberNM_002417
Gene Symbol KI67
Gene ID (NCBI) 4288
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP46013
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

背景介绍

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.

实验方案

Product Specific Protocols
FC protocol for Ki-67 antibody 98143-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols